Using a Formvar-Coated Bridge to Apply Formvar Support Film to TEM Grids
Using a Formvar-Coated Bridge to Apply Formvar Support Film to TEM Grids
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DOI：
10.1017/s1551929500064476
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发表时间：
1999-06
期刊：
影响因子：
--
通讯作者：
J. P. Shields
中科院分区：
文献类型：
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作者：
J. P. Shields
One conventional method for picking up ultrathin sections for transmission electron microscopy (TEM) is to prepare the mesh or slot grids with a formvar support film and then pick up your sections as they are floating in the knife boat. An alternative method is to first pick up sections on a naked grid and then lay them down on formvar film suspended over holes drilled in an aluminum bridge. I use this method when picking up serial sections on slot grids. A description of the method follows. The bridges I have were manufactured at the University of Georgia instrumentation Shop. They can also be purchased pre-made (for example, from SPI, Inc. They list them as Domino Racks), or one can make them from aluminum with a vise and drill. The sample bridge in the figure is similar to what I use. It is 5 cm long, 2.5 cm wide.
