IMMUNOPHENOTYPIC ANALYSIS OF INFILTRATING LEUKOCYTES AND MICROGLIA IN AN EXPERIMENTAL RAT GLIOMA
IMMUNOPHENOTYPIC ANALYSIS OF INFILTRATING LEUKOCYTES AND MICROGLIA IN AN EXPERIMENTAL RAT GLIOMA
复制标题
DOI：
10.1007/bf00299407
复制
发表时间：
1992-05-01
影响因子：
12.7
通讯作者：
STREIT, WJ
中科院分区：
文献类型：
--
作者：
MORIOKA, T;BABA, T;STREIT, WJ
主要组织相容性复合物（MHC），以及淋巴细胞和巨噬细胞抗原的外观和细胞分布进行了研究，在一个充分发展的实验大鼠前脑胶质瘤。在整个肿瘤中发现了表达CR3补体受体分子和MHC II类（Ia）抗原的活化的小胶质细胞和小胶质细胞衍生的巨噬细胞，并且沿着肿瘤的周边密度增加。MHC I类抗原的表达是完全不存在的肿瘤细胞，只有偶尔发现小胶质细胞。白细胞共同抗原，CD4和CD8抗原的表达在整个肿瘤中是明显的，并与淋巴细胞，血管周围细胞和小胶质细胞相关。表达ED 2巨噬细胞表位的细胞几乎完全是血管周围型的，并揭示了与Ia抗原阳性细胞不同的分布。ED 2表位零星发现的分枝小胶质细胞。结果显示，尽管血液单核细胞和CNS小胶质细胞严重浸润，但肿瘤没有表现出由炎性细胞引起的破坏的证据。肿瘤免疫抑制活性的可能机制，防止小胶质细胞和血液单核细胞的免疫激活。
The appearance and cellular distribution of major histocompatibility complex (MHC), as well as lymphocytic and macrophage antigens has been studied in a fully developed experimental rat forebrain glioma. Activated microglial cells and microglia-derived macrophages expressing CR3 complement receptor molecules and MHC class II (Ia) antigen were found throughout the tumor, and with increased density along the tumor's periphery. MHC class I antigen expression was entirely absent from tumor cells, and found only occasionally on microglia. The expression of leukocyte common antigen, and CD4 and CD8 antigens was conspicuous throughout the tumor, and associated with lymphocytes, perivascular cells, and microglia. Cells expressing the ED2 macrophage epitope were almost exclusively of the perivascular type and revealed a distribution dissimilar to that of cells positive for Ia antigen. The ED2 epitope was found sporadically on ramified microglial cells. The results show that despite heavy infiltration with blood mononuclear and CNS microglial cells, the tumor showed no evidence of destruction caused by inflammatory cells. Possible mechanisms of tumor immunosuppressive activity preventing the full immunological activation of microglia and blood mononuclear cells are discussed.