CELLULAR CONTROL OF SYNTHESIS AND ACTIVITY OF BACTERIAL LUMINESCENT SYSTEM
CELLULAR CONTROL OF SYNTHESIS AND ACTIVITY OF BACTERIAL LUMINESCENT SYSTEM
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DOI：
10.1128/jb.104.1.313-322.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
HASTINGS, JW
中科院分区：
文献类型：
--
作者：
NEALSON, KH;PLATT, T;HASTINGS, JW
在摇瓶中生长的生物发光细菌中，已经显示出在指数生长期间在相对短的爆发中合成酶萤光素酶。荧光素酶基因在新鲜接种的培养物中似乎完全失活;随后发生的荧光素酶优先合成的脉冲是其在脱氧核糖核酸转录水平上激活的结果，这归因于细胞生长对培养基的“调节”作用。虽然在基本培养基中生长的细胞也表现出类似的发光系统合成的爆发，但相对于细胞质量，合成的量在数量上较少。在这样的条件下，添加精氨酸导致生物发光的显著刺激。这归因于对现有合成模式的刺激，而不是诱导或去阻遏本身。
In bioluminescent bacteria growing in shake flasks, the enzyme luciferase has been shown to be synthesized in a relatively short burst during the period of exponential growth. The luciferase gene appears to be completely inactive in a freshly inoculated culture; the pulse of preferential luciferase synthesis which occurs later is the consequence of its activation at the level of deoxyribonucleic acid transcription which is attributed to an effect of a “conditioning” of the medium by the growing of cells. Although cells grown in a minimal medium also exhibit a similar burst of synthesis of the luminescent system, the amount of synthesis is quantitatively less, relative to cell mass. Under such conditions, added arginine results in a striking stimulation of bioluminescence. This is attributed to a stimulation of existing patterns of synthesis and not to induction or derepression per se.