Delta 9-tetrahydrocannabinol suppresses vomiting behavior and Fos expression in both acute and delayed phases of cisplatin-induced emesis in the least shrew.
Delta 9-tetrahydrocannabinol suppresses vomiting behavior and Fos expression in both acute and delayed phases of cisplatin-induced emesis in the least shrew.
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DOI：
10.1016/j.bbr.2008.07.028
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发表时间：
2009-01-03
影响因子：
2.7
通讯作者：
Darmani, Nissar A.
中科院分区：
文献类型：
--
作者：
Ray, Andrew P.;Griggs, Lisa;Darmani, Nissar A.
Cisplatin chemotherapy frequently causes severe vomiting in two temporally-separated clusters of bouts dubbed the acute and delayed phases. Cannabinoids can inhibit the acute phase, albeit through a poorly understood mechanism. We examined the substrates of cannabinoid-mediated inhibition of both emetic phases via immunolabeling for serotonin, Substance P, cannabinoid receptors 1 and 2 (CB1, CB2), and the neuronal activation marker Fos in the least shrew (Cryptotis parva). Shrews were injected with cisplatin (10 mg/kg ip), and one of vehicle, Δ9-THC, or both Δ9-THC and the CB1 receptor antagonist SR141716A (2 mg/kg ip), and monitored for vomiting. Δ9-THC-pretreatment caused concurrent decreases in the number of shrews expressing vomiting and Fos-immunoreactivity (Fos-IR), effects which were blocked by SR141716A-pretreatment. Acute phase vomiting induced Fos-IR in the solitary tract nucleus (NTS), dorsal motor nucleus of the vagus (DMNX), and area postrema (AP), whereas in the delayed phase Fos-IR was not induced in the AP at all, and was induced at lower levels in the other nuclei when compared to the acute phase. CB1 receptor-IR in the NTS was dense, punctate labeling indicative of presynaptic elements, which surrounded Fos-expressing NTS neurons. CB2 receptor-IR was not found in neuronal elements, but in vascular-appearing structures. All areas correlated with serotonin- and Substance P-IR. These results support published acute phase data in other species, and are the first describing Fos-IR following delayed phase emesis. The data suggest overlapping but separate mechanisms are invoked for each phase, which are sensitive to antiemetic effects of Δ9-THC mediated by CB1 receptors.
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DOI：
10.1016/j.molbrainres.2004.08.025
发表时间：
2004-12-06
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Golech, SA;McCarron, RM;Spatz, M
通讯作者：
Spatz, M
影响因子：
5
作者：
Darmani, NA;Johnson, JC
通讯作者：
Johnson, JC
影响因子：
3.6
作者：
Darmani, NA;Crim, JL
通讯作者：
Crim, JL
影响因子：
3.4
作者：
Kwiatkowska, M;Parker, LA;Mechoulam, R
通讯作者：
Mechoulam, R
影响因子：
2.9
作者：
Davis, SF;Derbenev, AV;Smith, BN
通讯作者：
Smith, BN