Germ cell transplantation as a potential biotechnological approach to fish reproduction
Germ cell transplantation as a potential biotechnological approach to fish reproduction
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DOI：
10.1007/s10695-012-9606-4
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发表时间：
2013-02-01
影响因子：
2.9
通讯作者：
Franca, L. R.
中科院分区：
文献类型：
--
作者：
Lacerda, S. M. S. N.;Costa, G. M. J.;Franca, L. R.
尽管生殖细胞移植在哺乳动物中的应用已经相对成熟，但该技术在进入 2000 年代后才适用于鱼类。在过去的十年中，已经开发了几种不同的方法，用于使用不同年龄和生命阶段的受体（例如囊胚期胚胎、新孵化的幼虫和性成熟标本）进行鱼类生殖细胞移植。由于生殖细胞可以通过成熟和受精过程发育成活生物体，鱼类生殖细胞移植开辟了生殖生物技术和水产养殖研究的新途径。例如，在鱼类中使用异种移植技术在保护濒危物种和利用代孕受体生产具有商业价值的鱼类方面取得了进展。此外，这还可以促进转基因鱼的工程设计。然而，与哺乳动物的情况一样，有关鱼类生殖干细胞的基本生物学和生理学知识仍然不完整，这对鱼类生殖细胞移植的应用造成了相当大的限制。进一步了解生殖干细胞将极大地促进鱼类生殖细胞移植的进步。
Although the use of germ cell transplantation has been relatively well established in mammals, the technique has only been adapted for use in fish after entering the 2000s. During the last decade, several different approaches have been developed for germ cell transplantation in fish using recipients of various ages and life stages, such as blastula-stage embryos, newly hatched larvae and sexually mature specimens. As germ cells can develop into live organisms through maturation and fertilization processes, germ cell transplantation in fish has opened up new avenues of research in reproductive biotechnology and aquaculture. For instance, the use of xenotransplantation in fish has lead to advances in the conservation of endangered species and the production of commercially valuable fish using surrogated recipients. Further, this could also facilitate the engineering of transgenic fish. However, as is the case with mammals, knowledge regarding the basic biology and physiology of germline stem cells in fish remains incomplete, imposing a considerable limitation on the application of germ cell transplantation in fish. Furthering our understanding of germline stem cells would contribute significantly to advances regarding germ cell transplantation in fish.