Expression of granulocyte colony‐stimulating factor receptor increases with differentiation in myeloid cells by a newly‐devised quantitative flow‐cytometric assay
Expression of granulocyte colony‐stimulating factor receptor increases with differentiation in myeloid cells by a newly‐devised quantitative flow‐cytometric assay
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通过新设计的定量流式细胞术检测粒细胞集落刺激因子受体的表达随着骨髓细胞的分化而增加
DOI：
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发表时间：
1995
影响因子：
6.5
通讯作者：
R. Ohno
中科院分区：
文献类型：
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作者：
K. Shinjo;A. Takeshita;K. Ohnishi;R. Ohno
总结。为了建立人或小鼠细胞上粒细胞集落刺激因子（G - CSF）受体的非同位素定量分析，我们设计了一种流式细胞术，使用生物素标记的G - CSF （b - G - CSF）和链霉亲和素- RED670偶联物染色的细胞。为了量化，我们应用Kolmogorov - Smirnov检验并计算了D值。根据b - G - CSF与G - CSF受体特异性结合导致的荧光强度的增加，从两个荧光谱的位移程度来评估D值。通过放射性同位素结合试验获得的G - CSF受体数量与通过流式细胞术测定的D值计算的数量之间存在良好的相关性。然后，研究了G - CSF受体在人骨髓细胞、外周血粒细胞和急性髓性白血病（AML）患者胚细胞上的表达。G‐CSF受体在CD34+CD33−、CD34+CD33+和CD34−CD33+细胞上的表达顺序如下：CD34 CD33+CD34+CD33+ CD33+CD34+CD33细胞上表达，表明受体随着成熟而增加。骨髓中CD34 - CD33+细胞的受体水平明显低于外周血中CD34 - CD33+细胞的受体水平。另一方面，在AML母细胞中发现G - CSF受体的异常表达模式。
Summary. In order to develop a non‐isotopic quantitative assay of granulocyte colony‐stimulating factor (G‐CSF) receptors on human or murine cells, we devised a flow‐cytometric assay using cells stained with biotin‐labelled G‐CSF (b‐G‐CSF) and a streptavidin‐RED670 conjugate. For quantification, we applied the Kolmogorov‐Smirnov test and calculated the D value. The D value was evaluated from the degree of shift in two fluorescence profiles according to the increase of fluorescence intensity due to the specific binding of b‐G‐CSF to G‐CSF receptors. A good correlation was observed between the number of G‐CSF receptors obtained by the radioisotopic binding assay and the number calculated from the D value by the flow‐cytometric assay. Then, expression of G‐CSF receptors on human bone marrow cells, peripheral blood granulocytes and blast cells from patients with acute myeloid leukaemia (AML) were studied. G‐CSF receptors was expressed on CD34+CD33−, CD34+CD33+ and CD34−CD33+ cells in the following order: CD34 CD33+CD34+CD33+CD34+CD33 cells, indicating that the receptors increased with maturation. The receptor levels of CD34−CD33+ cells in bone marrow were apparently lower than those of CD34−CD33+ cells in peripheral blood granulocytes. On the other hand, an abnormal expression pattern of G‐CSF receptors was noted in AML blast cells.
细胞系和原发性白血病细胞上的白细胞介素 3、GM-CSF 和 G-CSF 受体表达：受体异质性及其与生长因子反应性的关系。
影响因子：
20.3
作者：
Park,LS;Waldron,PE;Friend,D;Sassenfeld,HM;Price,V;Anderson,D;Cosman,D;Andrews,RG;Bernstein,ID;Urdal,DL
通讯作者：
Urdal,DL
影响因子：
56.9
作者：
SOUZA, LM;BOONE, TC;WELTE, K
通讯作者：
WELTE, K