Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles
Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles
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DOI：
10.1016/s0378-4274(98)00151-9
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发表时间：
1998-11-12
影响因子：
3.5
通讯作者：
Rahman, Q
中科院分区：
文献类型：
--
作者：
Afaq, F;Abidi, P;Rahman, Q
青石棉、温石棉纤维和超细二氧化钛 (UF-TiO2) 颗粒的细胞毒性和氧化反应在大鼠肺泡巨噬细胞 (AM) 和外周红细胞 (RBC) 中进行单次气管内暴露 (5 mg) 30 天后测量。纤维/颗粒滴注后一个月观察到以下反应： (1) AM 数量增加； (2)无细胞肺灌洗液中乳酸脱氢酶和酸性磷酸酶活性升高； (3)AM和外周红细胞中与过氧化氢或硫代巴比妥酸反应的物质均升高； (4)AM和外周红细胞中谷胱甘肽过氧化物酶、谷胱甘肽还原酶和过氧化氢酶均发生改变； (5)AM和外周红细胞中谷胱甘肽和抗坏血酸均下降。两个纤维暴露组的所有反应以及 UF-TiO2 暴露组的大多数反应均与阴性对照存在显着差异。对三种测试物质的反应水平表明毒性的递减顺序为青石棉 > 温石棉 > UF-TiO2。 (C) 1998 Elsevier Science Ireland Ltd. 保留所有权利。
The cytotoxic and oxidative responses of crocidolite and chrysotile asbestos fibers and ultrafine titanium dioxide (UF-TiO2) particles were measured in alveolar macrophages (AM) and peripheral red blood cells (RBC) of rat after 30 days with a single intratracheal exposure (5 mg). The following responses were observed one month after fiber/particle instillation: (1) AM population increased; (2) lactate dehydrogenase and acid phosphatase activities in cell free lung lavage fluid increased; (3) substances that react with hydrogen peroxide or thiobarbituric acid were elevated in both AM and peripheral RBC; (4) glutathione peroxidase, glutathione reductase, and catalase were altered in both AM and peripheral RBC; (5) glutathione and ascorbic acid decreased in both AM and peripheral RBC. A significant difference from negative controls was noted in all responses of the two fiber-exposed groups, and in most responses of the UF-TiO2-exposed group. The level of responses to the three test substances suggested a decreasing order of toxicity, with crocidolite > chrysotile > UF-TiO2. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.