QTL analyses and comparative genetic mapping of frost tolerance, winter survival and drought tolerance in meadow fescue (Festuca pratensis Huds.)
QTL analyses and comparative genetic mapping of frost tolerance, winter survival and drought tolerance in meadow fescue (Festuca pratensis Huds.)
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DOI：
10.1007/s00122-011-1590-z
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发表时间：
2011-08-01
影响因子：
5.4
通讯作者：
Rognli, Odd Arne
中科院分区：
文献类型：
--
作者：
Alm, Vibeke;Busso, Carlos S.;Rognli, Odd Arne
对草地羊茅(Festuca Pratensis Huds)的耐霜性、抗旱性和越冬存活率的数量性状基因座进行了定位。并与小麦和水稻的相应性状进行比较，研究与已知的非生物耐逆基因和推测的同源QTL的共位。禾本科植物的基因组是高度大向同源的；然而，高羊茅/黑麦属和小麦亚科的同源染色体4和5显示出重大的结构差异，这在耐霜性的比较基因组学中特别有趣。高羊茅5F染色体上的两个耐霜性/越冬性QTL的位置很可能与小麦同源群5A染色体上的Fr-A1和Fr-A2座位相对应。位于3F染色体上的一个长期抗旱性QTL(与水稻1号同源)支持从高羊茅基因组片段向同源黑麦草染色体(3L)的导入，证明该基因组区域是抗旱胁迫的极佳来源。高羊茅中的几个耐逆QTL与小麦科中的QTL和基因，特别是脱水蛋白、CBF转录因子和春化反应基因的位置一致，表明结构基因或调控基因在进化上较远的物种中具有保守的作用。
Quantitative trait loci (QTLs) for frost and drought tolerance, and winter survival in the Weld, were mapped in meadow fescue (Festuca pratensis Huds.) and compared with corresponding traits in Triticeae and rice to study co-location with putatively orthologous QTLs and known abiotic stress tolerance genes. The genomes of grass species are highly macrosyntenic; however, the Festuca/Lolium and Triticeae homoeologous chromosomes 4 and 5 show major structural differences that is especially interesting in comparative genomics of frost tolerance. The locations of two frost tolerance/winter survival QTLs on Festuca chromosome 5F correspond most likely to the Fr-A1 and Fr-A2 loci on wheat homoeologous group 5A chromosomes. A QTL for long-term drought tolerance on chromosome 3F (syntenic with rice 1) support evidence from introgression of Festuca genome segments onto homoeologous Lolium chromosomes (3L) that this genome region is an excellent source of tolerance towards drought stress. The coincident location of several stress tolerance QTL in Festuca with QTL and genes in Triticeae species, notably dehydrins, CBF transcription factors and vernalisation response genes indicate the action of structural or regulatory genes conserved across evolutionarily distant species.