2D NMR Trace Analysis by Continuous Hyperpolarization at High Magnetic Field
2D NMR Trace Analysis by Continuous Hyperpolarization at High Magnetic Field
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DOI：
10.1002/anie.201507831
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发表时间：
2015-11-23
影响因子：
16.6
通讯作者：
Tessari, Marco
中科院分区：
文献类型：
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作者：
Eshuis, Nan;Aspers, Ruud L. E. G.;Tessari, Marco
核磁共振是化学分析中常用的技术，因为它对分子结构的敏感性、定量特性和简单的样品制备。然而，复杂混合物中痕量分析物的测定通常受到低灵敏度和广泛的信号重叠的限制。在这里，我们提出了一种基于可逆交换（SABRE）信号放大的高磁场连续超极化方法，可以直接纳入多维核磁共振实验。该方法在二维相关实验中实现，允许在复杂溶液中检测和定量纳摩尔浓度的分析物。
Nuclear magnetic resonance is often the technique of choice in chemical analysis because of its sensitivity to molecular structure, quantitative character, and straightforward sample preparation. However, determination of trace analytes in complex mixtures is generally limited by low sensitivity and extensive signal overlap. Here, we present an approach for continuous hyperpolarization at high magnetic field that is based on signal amplification by reversible exchange (SABRE) and can be straightforwardly incorporated in multidimensional NMR experiments. This method was implemented in a 2D correlation experiment that allows detection and quantification of analytes at nanomolar concentration in complex solutions.