Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis.
Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis.
复制标题
时钟组件 OsPRR73 通过调节 OsHKT2;1 介导的钠稳态来正向调节大米耐盐性
DOI：
10.15252/embj.2020105086
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发表时间：
2021-02-01
期刊：
影响因子：
--
通讯作者：
Wang L
中科院分区：
文献类型：
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作者：
Wei H;Wang X;He Y;Xu H;Wang L
生物钟在水稻耐盐性中的作用尚不完全清楚。在此，我们表明，在OsPRR（水稻假反应调节子）家族成员中，OsPRR 73特异性地赋予水稻耐盐性。值得注意的是，osprr 73无效突变体的籽粒大小和产量显着下降，在盐胁迫的存在下，积累了较高水平的活性氧和钠离子。RNA测序和生物化学分析鉴定OsHKT 2;1是OsPRR 73介导耐盐性的转录靶点，OsHKT 2; 1编码质膜定位的Na+转运蛋白。相应地，OsHKT 2;1的无效突变体表现出对盐胁迫的耐受性增加。免疫沉淀-质谱（IP-MS）分析进一步鉴定HDAC 10为OsPRR 73的核相互作用物和OsHKT 2的共阻遏物;1.因此，OsHKT 2;1启动子区的H3 K9 ac组蛋白标记在osprr 73突变体中显著减少。总之，我们的研究结果表明，盐诱导的OsPRR 73表达通过招募HDAC 10来转录抑制OsHKT 2;1，从而减少细胞Na+积累而赋予盐耐受性。这证实了生物钟成分与水稻耐盐性之间的一种新的分子联系。水稻伪反应调节因子（PRR）家族的一个成员在盐胁迫下通过染色质修饰诱导降低钠转运蛋白的表达。
The roles of clock components in salt stress tolerance remain incompletely characterized in rice. Here, we show that, among OsPRR (Oryza sativa Pseudo‐Response Regulator) family members, OsPRR73 specifically confers salt tolerance in rice. Notably, the grain size and yield of osprr73 null mutants were significantly decreased in the presence of salt stress, with accumulated higher level of reactive oxygen species and sodium ions. RNA sequencing and biochemical assays identified OsHKT2;1, encoding a plasma membrane‐localized Na+ transporter, as a transcriptional target of OsPRR73 in mediating salt tolerance. Correspondingly, null mutants of OsHKT2;1 displayed an increased tolerance to salt stress. Immunoprecipitation‐mass spectrometry (IP‐MS) assays further identified HDAC10 as nuclear interactor of OsPRR73 and co‐repressor of OsHKT2;1. Consistently, H3K9ac histone marks at OsHKT2;1 promoter regions were significantly reduced in osprr73 mutant. Together, our findings reveal that salt‐induced OsPRR73 expression confers salt tolerance by recruiting HDAC10 to transcriptionally repress OsHKT2;1, thus reducing cellular Na+ accumulation. This exemplifies a new molecular link between clock components and salt stress tolerance in rice. A member of the pseudo response regulator (PRR) family in rice is induced upon salt stress to decrease expression of a sodium transporter via chromatin modifications.