Characterization of transcriptomic response in ovules derived from inter-subgeneric hybridization in Prunus (Rosaceae) species
Characterization of transcriptomic response in ovules derived from inter-subgeneric hybridization in Prunus (Rosaceae) species
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DOI：
10.1007/s00497-021-00423-2
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发表时间：
2021-06
影响因子：
3.4
通讯作者：
T. Morimoto;Y. Kitamura;Koji Numaguchi;A. Itai
中科院分区：
文献类型：
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作者：
T. Morimoto;Y. Kitamura;Koji Numaguchi;A. Itai
关键信息李属杂交种子失败的表征提供了对植物物种中保守的或谱系特异性的杂交不相容性机制的深入了解。摘要不同物种之间杂交导致的合子后杂交不相容性涉及在不同发育阶段发生的复杂机制。胚胎停滞，随后是种子败育，是这种不亲和反应的第一阶段，并抑制杂种种子的发育。在蔷薇科木本植物李属中，一些种间杂交导致果实发育早期阶段落果，其中种子发育不良可能是观察到的杂种不亲和性的原因。在这项研究中，我们研究了李属亚属间杂交中的胚珠发育和发育中胚珠的转录组。我们对梅花（李子亚属）进行了杂交，由梅授粉。桃（杏仁亚属），发现在落果前胚珠和种皮发生退化。转录组分析鉴定出富含多种 GO 途径的差异表达基因，包括细胞器发育、刺激反应和信号传导。其中，细胞器相关基因在胚珠发育过程中受到主动调控，在种间杂交早期表达量较高，后期表达量下降，表明细胞器功能的差异性调控可能导致杂种胚珠的退化。此外，与胚珠和种皮发育相关的基因，例如编码AGL样和生长素反应的基因，在李属种间杂交中受到差异性调节。我们的结果提供了李属杂交种子败育的组织学和分子信息，可用于开发新的杂交作物。此外，我们还比较和讨论了李属和其他植物物种对杂交种子失败的转录组反应，这为了解某些植物物种中保守的或谱系特异性的杂交不相容机制提供了见解。
Key messageCharacterization of hybrid seed failure inPrunusprovides insight into conserved or lineage-specific hybrid incompatibility mechanisms in plant species.AbstractPostzygotic hybrid incompatibility resulting from a cross between different species involves complex mechanisms occurring at various developmental stages. Embryo arrest, followed by seed abortion, is the first stage of such incompatibility reactions and inhibits hybrid seed development. InPrunus, a rosaceous woody species, some interspecific crosses result in fruit drop during the early stage of fruit development, in which inferior seed development may be accounted for the observed hybrid incompatibility. In this study, we investigated ovule development and the transcriptomes of developing ovules in inter-subgeneric crosses ofPrunus. We conducted a cross ofPrunus mume(subgenusPrunus), pollinated byP. persica(subgenusAmygdalus), and found that ovule and seed coat degeneration occurs before fruit drop. Transcriptome analysis identified differentially expressed genes enriched in several GO pathways, including organelle development, stimulus response, and signaling. Among these pathways, the organelle-related genes were actively regulated during ovule development, as they showed higher expression in the early stage of interspecific crosses and declined in the later stage, suggesting that the differential regulation of organelle function may induce the degeneration of hybrid ovules. Additionally, genes related to ovule and seed coat development, such as genes encodingAGL-like and auxin response, were differentially regulated inPrunusinterspecific crosses. Our results provide histological and molecular information on hybrid seed abortion inPrunusthat could be utilized to develop new hybrid crops. Additionally, we compared and discussed transcriptome responses to hybrid seed failure inPrunusand other plant species, which provides insight into conserved or lineage-specific hybrid incompatibility mechanisms in some plant species.