Differential gene expression in laboratory strains of human head and body lice when challenged with Bartonella quintana, a pathogenic bacterium.
Differential gene expression in laboratory strains of human head and body lice when challenged with Bartonella quintana, a pathogenic bacterium.
复制标题
人类头虱和体虱实验室菌株在受到致病菌五体巴尔通体攻击时的差异基因表达。
DOI：
10.1111/imb.12077
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发表时间：
2014
影响因子：
2.6
通讯作者：
Pittendrigh,BR
中科院分区：
文献类型：
--
作者：
Previte,D;Olds,BP;Yoon,K;Sun,W;Muir,W;Paige,KN;Lee,SH;Clark,J;Koehler,JE;Pittendrigh,BR
Human head and body lice are obligatory hematophagous ectoparasites that belong to a single species,Pediculus humanus. Only body lice, however, are vectors of the infectious Gram‐negative bacteriumBartonella quintana. Because of their near identical genomes, yet differential vector competence, head and body lice provide a unique model system to study the gain or loss of vector competence. Using ourin vitrolouse‐rearing system, we infected head and body lice with blood containingB. quintanain order to detect both differences in the proliferation ofB. quintanaand transcriptional differences of immune‐related genes in the lice.B. quintanaproliferated rapidly in body lice at 6 days post‐infection, but plateaued in head lice at 4 days post‐infection. RNAseq and quantitative real‐time PCR validation analyses determined gene expression differences. Eight immunoresponse genes were observed to be significantly different with many associated with the Toll pathway: Fibrinogen‐like protein, Spaetzle, Defensin 1, Serpin, Scavenger receptor A and Apolipoporhrin 2. Our findings support the hypothesis that body lice, unlike head lice, fight infection fromB. quintanaonly at the later stages of its proliferation.
