Altered diversity and composition of the gut microbiome in patients with cervical cancer
Altered diversity and composition of the gut microbiome in patients with cervical cancer
复制标题
宫颈癌患者肠道微生物组的多样性和组成发生改变
DOI：
10.1186/s13568-019-0763-z
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发表时间：
2019-03-23
期刊：
影响因子：
3.7
通讯作者：
Yuan, Zhiyong
中科院分区：
文献类型：
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作者：
Wang, Zhongqiu;Wang, Qingxin;Yuan, Zhiyong
肠道微生物群与许多人类疾病的发展有关，包括消化系统疾病和非消化系统疾病。在这项研究中，我们通过16S rRNA测序分析研究了宫颈癌（CCa）患者与健康对照组的肠道细菌是否存在差异。研究对象包括8名CCa和5名健康对照。采用16S rRNA V4可变区的PCR扩增和Illumina HiSeq平台的深度测序，对粪便DNA中的微生物群进行了分析。cca相关肠道菌群α多样性呈增加趋势，但未达到统计学显著性。通过β多样性分析，群落结构的组间变异性表明癌症患者和健康对照者之间存在明显的差异。患者和对照组的肠道菌群谱不同；即，变形杆菌在CCa患者中的比例显著高于其他患者（ρ = 0.012）。7个属与对照组的相对丰度差异显著（均ρ < 0.05），包括escherichia - shigella、Roseburia、Pseudomonas、Lachnoclostridium、Lachnospiraceae_UCG-004、DoreaandSuccinivibrio。通过线性判别分析效应大小（LEfSe）确定了CCa患者的特征微生物组。变形杆菌门、副杆菌属、埃希氏菌属、希氏菌属和玫瑰杆菌属可能为CCa提供新的潜在生物标志物。综上所述，这是第一个关于CCa患者肠道微生物群的研究，并证明了显著改变的多样性和组成。
Gut microbiota have been implicated in the development of many human diseases, including both digestive diseases and non-digestive diseases. In this study, we investigated whether the gut bacteria differed in cervical cancer (CCa) patients compared with healthy controls by 16S rRNA sequencing analysis. Subjects including eight CCa and five healthy controls were included. Microbiota profiles in fecal DNA were characterized by PCR amplification of the 16S rRNA V4 variable region and deep sequencing using the Illumina HiSeq platform. The CCa-associated gut microbiota had an increasing trend in alpha diversity, although statistical significance was not reached. Inter-group variability in community structure by beta diversity analysis showed a clear separation between cancer patients and healthy controls. Gut microbiota profiles were different between patients and controls; namely, the proportions ofProteobacteriaphylum was notably higher in patients with CCa (ρ = 0.012). Seven genera differentiated significantly in relative abundance between CCa and controls (all ρ < 0.05), includingEscherichia–Shigella,Roseburia,Pseudomonas,Lachnoclostridium,Lachnospiraceae_UCG-004,DoreaandSuccinivibrio. The characteristic microbiome in CCa patients was also identified by linear discriminant analysis effect size (LEfSe). The phylumProteobacteria, and the genusParabacteroides, Escherichia_ShigellsandRoseburiamay provide novel potential biomarkers for CCa. Taken together, this is the first study on gut microbiota in patients with CCa, and demonstrated the significantly altered diversity and composition.