Reduced Influenza B-Specific Postvaccination Antibody Cross-reactivity in the B/Victoria Lineage-Predominant 2019/20 Season.
Reduced Influenza B-Specific Postvaccination Antibody Cross-reactivity in the B/Victoria Lineage-Predominant 2019/20 Season.
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2019/20 年 B 型/维多利亚谱系为主季节的 B 型流感特异性疫苗接种后抗体交叉反应性降低。
DOI：
10.1093/cid/ciaa1481
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Wan,Xiu-Feng
中科院分区：
文献类型：
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作者：
Xie,Hang;Xiang,Ruoxuan;Wan,HamiltonJ;Plant,EwanP;Radvak,Peter;Kosikova,Martina;Li,Xing;Zoueva,Olga;Ye,Zhiping;Wan,Xiu-Feng
背景美国2019/20流感季节的流感活跃度仍然较高且广泛，早期流感季节以B型病毒为主。大多数 B 病毒属于 B/Victoria (B/Vic) 谱系，并在血凝素 (3DEL) 中含有氨基酸 (aa) 162-164 三重缺失。这些 3DEL 病毒在抗原上与 B/Colorado/06/2017 (CO/06) 不同，后者是 2018/19 和 2019/20 季节的 B/Vic 疫苗成分，代表血凝素 (2DEL) 中氨基酸 162-163 双重缺失的病毒。方法我们对循环的 B/Vic 病毒进行了分子表征和系统发育分析。我们还使用从接种不同类型 2018/19 或 2019/20 季节性疫苗的健康受试者收集的存档人类疫苗接种后血清进行血凝抑制 (HAI) 测定。分析了它们与代表性3DEL病毒的HAI交叉反应性。结果CO/06特异性人类疫苗接种后血清在针对疫苗类型进行调整后，显着降低了HAI与代表性3DEL病毒的交叉反应性，尤其是136E+150K亚组。与针对 136E+150N 病毒的几何平均滴度相比，针对含有 136E+150K 突变的 3DEL 病毒的几何平均滴度在所有人群中低 1.6 倍（P= .051），在成人中低 1.9 倍（P= .016）。结论我们的结果表明，2019/20 流感季节 B/Vic 疫苗成分诱导的疫苗接种后抗体减少了 HAI 与美国主要 3DEL 病毒的交叉反应。如果 3DEL 136E+150K 亚组卷土重来并在 2020/21 流感季节占据主导地位，则有必要密切监测该亚组。
BackgroundThe influenza activity of the 2019/20 season remained high and widespread in the United States with type B viruses predominating the early season. The majority of B viruses characterized belonged to B/Victoria (B/Vic) lineage and contained a triple deletion of amino acid (aa) 162–164 in hemagglutinin (3DEL). These 3DEL viruses are antigenically distinct from B/Colorado/06/2017 (CO/06)—the B/Vic vaccine component of the 2018/19 and 2019/20 seasons representing the viruses with a double deletion of aa 162–163 in hemagglutinin (2DEL).MethodsWe performed molecular characterization and phylogenetic analysis of circulating B/Vic viruses. We also conducted hemagglutination inhibition (HAI) assay using archived human postvaccination sera collected from healthy subjects administered with different types of 2018/19 or 2019/20 seasonal vaccines. Their HAI cross-reactivity to representative 3DEL viruses was analyzed.ResultsThe CO/06-specific human postvaccination sera, after being adjusted for vaccine type, had significantly reduced HAI cross-reactivity toward representative 3DEL viruses, especially the 136E+150K subgroup. The geometric mean titers against 3DEL viruses containing 136E+150K mutations were 1.6-fold lower in all populations (P= .051) and 1.9-fold lower in adults (P= .016) compared with those against the 136E+150N viruses.ConclusionsOur results indicate that postvaccination antibodies induced by the B/Vic vaccine component of the 2019/20 influenza season had reduced HAI cross-reactivity toward predominant 3DEL viruses in the United States. A close monitoring of the 3DEL 136E+150K subgroup is warranted should this subgroup return and predominate the 2020/21 influenza season.