Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells.
Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells.
复制标题
DOI：
10.1186/scrt19
复制
发表时间：
2010-06-29
影响因子：
7.5
通讯作者：
Bunnell BA
中科院分区：
文献类型：
--
作者：
Gimble JM;Guilak F;Bunnell BA
脂肪组织现在被认为是一种可获取、丰富且可靠的分离成体干细胞的场所，适用于组织工程和再生医学应用。过去十年见证了与体外和动物模型中新鲜分离的基质血管部分细胞和贴壁、培养扩增、脂肪来源的基质/干细胞的分离、表征、冷冻保存、分化和移植相关的临床前数据的激增。这项工作为支持脂肪源细胞在安全性和有效性试验中的临床转化应用提供了证据。本文回顾了迄今为止在胃肠病学、神经病学、骨科、重建外科和相关临床学科领域已发表的使用自体脂肪来源细胞的病例报告和 I-III 期临床证据。讨论和评估了该领域未来的方向和面临的挑战。
Adipose tissue is now recognized as an accessible, abundant, and reliable site for the isolation of adult stem cells suitable for tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models. This body of work has provided evidence supporting clinical translational applications of adipose-derived cells in safety and efficacy trials. The present article reviews the case reports and phase I-III clinical evidence using autologous adipose-derived cells that have been published, to date, in the fields of gastroenterology, neurology, orthopedics, reconstructive surgery, and related clinical disciplines. Future directions and challenges facing the field are discussed and evaluated.