Sensitive detection of prostatic hematogenous tumor cell dissemination using prostate specific antigen and prostate specific membrane-derived primers in the polymerase chain reaction.
Sensitive detection of prostatic hematogenous tumor cell dissemination using prostate specific antigen and prostate specific membrane-derived primers in the polymerase chain reaction.
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在聚合酶链式反应中使用前列腺特异性抗原和前列腺特异性膜衍生引物灵敏检测前列腺血行肿瘤细胞传播。
DOI：
10.1016/s0022-5347(01)67651-1
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Fair,WR
中科院分区：
文献类型：
--
作者：
Israeli,RS;MillerJr,WH;Su,SL;Samadi,DS;Powell,CT;Heston,WD;Wise,GJ;Fair,WR
我们开发了一种基于聚合酶链反应的检测方法，可以灵敏地检测前列腺癌患者的血行性肿瘤细胞播散。我们进行了“巢式聚合酶链反应”，扩增前列腺特异性抗原（PSA）和前列腺特异性膜抗原特有的信使核糖核酸序列，并比较了各自的结果。PSA引物聚合酶链反应检测到前列腺肿瘤细胞2例（6.7%），前列腺特异性膜引物检测到肿瘤细胞19例（63.3%）。16例阴性对照均有PSA和前列腺特异性膜聚合酶链反应阴性。重复实验以确认结果，聚合酶链反应产物通过脱氧核糖核酸测序和Southern分析进行验证。通过前列腺特异性膜而非PSA聚合酶链反应检测到循环前列腺肿瘤细胞的患者包括13名接受根治性前列腺切除术的患者中的7名，他们在该检测时血清PSA水平无法测量。这些发现对未来疾病复发和进展的意义将被调查。
We developed a polymerase chain reaction based assay enabling sensitive detection of hematogenous tumor cell dissemination in patients with prostate cancer. We performed" nested polymerase chain reaction," amplifying messenger ribonucleic acid sequences unique to prostate specific antigen (PSA) and to the prostate specific membrane antigen, and compared the respective results. Prostatic tumor cells were detected in 2 of 30 patients (6.7%) by polymerase chain reaction with PSA derived primers, while prostate specific membrane primers detected tumor cells in 19 (63.3%). All 16 negative controls had negative PSA and prostate specific membrane polymerase chain reaction. Assays were repeated to confirm results, and polymerase chain reaction products were verified by deoxyribonucleic acid sequencing and Southern analysis. Patients harboring circulating prostatic tumor cells as detected by prostate specific membrane and not by PSA polymerase chain reaction included 7 of 13 previously treated by radical prostatectomy who had nonmeasurable serum PSA levels at the time of this assay. The significance of these findings with respect to future disease recurrence and progression will be investigated.