Cell adhesion and integrin expression are modulated by oxidative stress in EA.hy 926 cells
Cell adhesion and integrin expression are modulated by oxidative stress in EA.hy 926 cells
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DOI：
10.1080/10715760701390027
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Aubery, Michele
中科院分区：
文献类型：
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作者：
Lamari, Foudil;Braut-Boucher, Francoise;Aubery, Michele
用EA研究了氧化应激对整合素介导的细胞与细胞外基质（ECM）粘附及相关凋亡的影响。hy 926内皮细胞用两种氧化剂处理（或不处理）：次黄嘌呤/黄嘌呤氧化酶系统（HX/XO）或叔丁基过氧化氢（t-BHP），这两种氧化剂都增加细胞凋亡。在低浓度的HX/XO（高达5 mU/ml）或t-BHP（高达125 mM）下，细胞粘附到玻连蛋白（Vn）和纤连蛋白（Fn）上增加，并阻止ROS诱导的凋亡。流式细胞仪分析整合素表达结果显示整合素α v和α 5亚基的表达分别增加和减少。使用功能阻断单克隆抗体对整合素亚基的细胞粘附抑制实验表明，α v β 1和α v β 3整合素参与细胞粘附到VN，和avb 3整合素在氧化剂处理的细胞中发挥了重要作用。对于粘附Fn，a5 b1和avb 1整合素所需的氧化剂处理的细胞。两者合计，结果表明，活性氧（ROS）产生的HX/ XO或t-BHP可以影响表达和/或激活的特定整合素的EA.hy926细胞与ECM的相互作用。
The effects of oxidative stress on integrin- mediated cell adhesion to the extracellular matrix ( ECM) and related apoptosis were investigated using the EA. hy926 endothelial cells treated ( or not) with two oxidants: the hypoxanthine/xanthine oxidase system (HX/XO) or the tert-butyl hydroperoxide (t-BHP) which both increased cell apoptosis. Cell adhesion onto vitronectin (Vn) and fibronectin (Fn) was increased at low concentrations of HX/XO (up to 5 mU/ml) or t-BHP (up to 125mM) and prevented ROS-induced apoptosis. Flow cytometry analysis of integrin expression showed that the expression of integrin av and alpha 5 subunits was, respectively, increased and decreased. Cell adhesion inhibition experiments using function-blocking monoclonal antibodies against integrin subunits indicated that alpha v beta 1 and alpha v beta 3 integrins were involved in adhesion of cells to Vn, and avb3 integrin played a major role in oxidant-treated cells. For adhesion to Fn, a5b1 and avb1 integrins were required for oxidant-treated cells. Taken together, the results suggest that reactive oxygen species ( ROS) produced either by HX/ XO or t-BHP could affect expression and/or activation of specific integrins in the interaction of EA.hy926 cells with ECM.