Surface sensing and lateral subcellular localization of WspA, the receptor in a chemosensory-like system leading to c-di-GMP production.
Surface sensing and lateral subcellular localization of WspA, the receptor in a chemosensory-like system leading to c-di-GMP production.
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WSPA的表面传感和外侧亚细胞定位，这是化学感应系统中的受体，导致C-DI-GMP产生。
DOI：
10.1111/mmi.12013
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发表时间：
2012-11
影响因子：
3.6
通讯作者：
Harwood CS
中科院分区：
文献类型：
--
作者：
O'Connor JR;Kuwada NJ;Huangyutitham V;Wiggins PA;Harwood CS
铜绿假单胞菌对琼脂表面的生长产生反应，产生环状二核苷酸，从而刺激生物膜的形成。这是由称为WSP的另一种细胞功能趋化样系统介导的。受体蛋白WSPA，在生物信息学上与甲基接受趋化蛋白没有区别。然而，与标准的化学感受器不同，WSPA不会在细胞极点形成稳定的簇。相反，它在极地和侧向亚细胞位置形成动态簇。为了研究WSP对表面反应的信号转导机制，我们对WSPA的结构和功能进行了研究，发现其C末端对其侧向亚细胞定位和功能具有重要作用。当这个区域被氨基酸的化学感受器取代时，WSPA变得两极局部化。此外，在WSPA的C-末端区域引入突变，使该蛋白能够形成更稳定的受体-受体相互作用，也导致WSPA蛋白激活信号转导的能力降低。具有WSpA C末端和N末端周质结构域的受体嵌合体来自感知氨基酸或苹果酸的化学感受器，它们与表面反应产生c-di-GMP。因此，WSP系统对表面的反应不需要保守WSPA周质区域的氨基酸序列。
Pseudomonas aeruginosa responds to growth on agar surfaces to produce cyclic-di-GMP, which stimulates biofilm formation. This is mediated by an alternative cellular function chemotaxis-like system called Wsp. The receptor protein WspA, is bioinformatically indistinguishable from methyl-accepting chemotaxis proteins. However, unlike standard chemoreceptors, WspA does not form stable clusters at cell poles. Rather, it forms dynamic clusters at both polar and lateral subcellular locations. To begin to study the mechanism of Wsp signal transduction in response to surfaces, we carried out a structure-function study of WspA and found that its C-terminus is important for its lateral subcellular localization and function. When this region was replaced with that of a chemoreceptor for amino acids, WspA became polarly localized. In addition, introduction of mutations in the C-terminal region of WspA that rendered this protein able to form more stable receptor-receptor interactions, also resulted in a WspA protein that was less capable of activating signal transduction. Receptor chimeras with a WspA C-terminus and N-terminal periplasmic domains from chemoreceptors that sense amino acids or malate responded to surfaces to produce c-di-GMP. Thus the amino acid sequence of the WspA periplasmic region did not need to be conserved for the Wsp system to respond to surfaces.
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DOI：
10.1073/pnas.0402638101
发表时间：
2004-06-08
影响因子：
11.1
作者：
Gitai, Z;Dye, N;Shapiro, L
通讯作者：
Shapiro, L
影响因子：
3.6
作者：
Alley, MRK
通讯作者：
Alley, MRK
影响因子：
2.6
作者：
Hoang, TT;Kutchma, AJ;Schweizer, HP
通讯作者：
Schweizer, HP
DOI：
10.1073/pnas.0511090103
发表时间：
2006-02-21
影响因子：
11.1
作者：
Kulesekara, H;Lee, V;Lory, S
通讯作者：
Lory, S
DOI：
10.1073/pnas.092071899
发表时间：
2002-05-14
影响因子：
11.1
作者：
Ames, P;Studdert, CA;Parkinson, JS
通讯作者：
Parkinson, JS