Transplantation of placenta-derived mesenchymal stem cells enhances angiogenesis after ischemic limb injury in mice.
Transplantation of placenta-derived mesenchymal stem cells enhances angiogenesis after ischemic limb injury in mice.
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DOI：
10.1111/jcmm.12489
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发表时间：
2016-01
影响因子：
5.3
通讯作者：
Xie X
中科院分区：
文献类型：
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作者：
Xie N;Li Z;Adesanya TM;Guo W;Liu Y;Fu M;Kilic A;Tan T;Zhu H;Xie X
基于间充质干细胞的疗法已成为治疗外周动脉疾病的一种有前途的方法。本研究的目的是检查人胎盘来源的间充质干细胞（PMSC）对小鼠后肢缺血的潜在影响。 PMSCs 从人胎盘组织中分离出来，并通过流式细胞术进行表征。通过肌肉注射递送荧光染料（CM-DiI）标记的 PMSC，生成体内手术结扎诱导的小鼠肢体缺血模型。我们的数据表明，与对照组相比，PMSCs 治疗显着增强了缺血小鼠后肢的微血管密度，改善了血液灌注并减少了病理。进一步的免疫染色研究表明，注射的 PMSC 可以融入脉管系统并分化为内皮细胞和平滑肌细胞，从而增强缺血后肢的血管生成。这可能部分解释了 PMSC 治疗的有益效果。综上所述，我们发现 PMSC 治疗可能是通过增强血管生成来治疗小鼠后肢缺血性损伤的有效治疗方式。
Mesenchymal stem cell‐based therapy has emerged as a promising approach for the treatment of peripheral arterial disease. The purpose of this study was to examine the potential effects of human placenta‐derived mesenchymal stem cells (PMSCs) on mouse hindlimb ischemia. PMSCs were isolated from human placenta tissue and characterized by flow cytometry. An in vivo surgical ligation‐induced murine limb ischemia model was generated with fluorescent dye (CM‐DiI) labelled PMSCs delivered via intramuscular injection. Our data show that PMSCs treatment significantly enhanced microvessel density, improved blood perfusion and diminished pathologies in ischemic mouse hindlimbs as compared to those in the control group. Further immunostaining studies suggested that injected PMSCs can incorporate into the vasculature and differentiate into endothelial and smooth muscle cells to enhance angiogenesis in ischemic hind limbs. This may in part explain the beneficial effects of PMSCs treatment. Taken together, we found that PMSCs treatment might be an effective treatment modality for treatment of ischemia‐induced injury to mouse hind limbs by enhancement of angiogenesis.