Indigenous microbial community governs the survival of Escherichia coli O157:H7 in constructed wetlands.
Indigenous microbial community governs the survival of Escherichia coli O157:H7 in constructed wetlands.
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DOI：
10.1016/j.jenvman.2023.117524
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发表时间：
2023-02
影响因子：
8.7
通讯作者：
Nan Zhang;Chunling Liang;Peiying Kan;Jiannan Yangyao;D. Lu;Z. Yao;Huihui Gan;D. Zhu
中科院分区：
文献类型：
--
作者：
Nan Zhang;Chunling Liang;Peiying Kan;Jiannan Yangyao;D. Lu;Z. Yao;Huihui Gan;D. Zhu
大肠杆菌O157：H7（E. coliO157：H7）及其调控因子在自然环境中已被广泛研究。然而，关于E.大肠杆菌157：H7在人工环境中，特别是在废水处理设施。本研究通过污染实验探讨了大肠杆菌的存活模式。大肠杆菌157：H7及其主要控制因子。结果表明，E. coliO157：H7在CW中的生长时间较长。基质铵态氮和速效磷是影响E. CW中的大肠杆菌157：H7。尽管微生物α多样性的影响很小，但一些关键类群，如气单胞菌属、月形单胞菌属和草履虫属，控制着E.大肠杆菌157：H7。此外，原核生物群落对E. coliO157：H7比真核生物群落高。生物学特性对E. coliO157：H7比非生物因子更能促进CWs的生长。本研究全面揭示了E. coliO157：H7在CWs中的环境行为，这是一个必不可少的除了E. coliO157：H7，为污水处理过程中生物污染的防治提供了理论依据。
The survival pattern ofEscherichia coliO157:H7 (E. coliO157:H7) and its regulatory factors in natural environments have been widely studied. However, there is little information about the survival ofE. coliO157:H7 in artificial environments, especially in wastewater treatment facilities. In this study, a contamination experiment was performed to explore the survival pattern ofE. coliO157:H7 and its central control factors in two constructed wetlands (CWs) under different hydraulic loading rates (HLRs). The results showed that the survival time ofE. coliO157:H7 was longer in the CW under the higher HLR. Substrate ammonium nitrogen and available phosphorus were the main factors that influenced the survival ofE. coliO157:H7 in CWs. Despite the minimal effect of microbial α-diversity, some keystone taxa, such asAeromonas,Selenomonas, andParamecium, governed the survival ofE. coliO157:H7. In addition, the prokaryotic community had a more significant impact on the survival ofE. coliO157:H7 than the eukaryotic community. The biotic properties had a more substantial direct power on the survival ofE. coliO157:H7 than the abiotic factors in CWs. Collectively, this study comprehensively disclosed the survival pattern ofE. coliO157:H7 in CWs, which is an essential addition to the environmental behavior ofE. coliO157:H7, providing a theoretical basis for the prevention and control of biological contamination in wastewater treatment processes.