Long-term radical scavenging activity of AA-2G and 6-acyl-AA-2G against 1,1-diphenyl-2-picrylhydrazyl.
Long-term radical scavenging activity of AA-2G and 6-acyl-AA-2G against 1,1-diphenyl-2-picrylhydrazyl.
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AA-2G 和 6-酰基-AA-2G 对 1,1-二苯基-2-三硝基苯肼的长期自由基清除活性。
DOI：
10.1248/bpb.25.1503
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发表时间：
2002
影响因子：
2
通讯作者：
I. Yamamoto
中科院分区：
文献类型：
--
作者：
J. Takebayashi;A. Tai;I. Yamamoto
对抗坏血酸(AA)C-2位氧取代衍生物的自由基清除活性进行了化学计量学评价。用比色法研究了它们在酸性条件下与稳定自由基1，1-二苯基-2-苦基肼(DPPH)的反应。2-O-α-D-葡萄糖基-L抗坏血酸(AA-2G)和一系列抗坏血酸(6-6-O-acyl-2-O-alpha-D-glucopyranosyl-L-ascorbic-AA-2G)具有较强的清除自由基活性。与AA相比，AA-2G或6-酰基-AA-2G与DPPH的反应非常缓慢。然而，这些衍生物中的一个分子在实验结束时(2小时)消耗了大约三个DPPH自由基分子。相反，一个AA分子清除两个DPPH自由基，反应在很短的时间(~lt；10min)内结束。在长期反应中，AA-2G和6-酰基-AA-2G对自由基的猝灭效果优于AA。
Stoichiometric evaluation of the radical scavenging activity of O-substituted derivatives at the C-2 position of ascorbic acid (AA) was conducted. Their reaction with a stable radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH), under an acidic condition was assessed by the colorimetric method. 2-O-alpha-D-Glucopyranosyl-L-ascorbic acid (AA-2G) and a series of 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-ascorbic acids (6-Acyl-AA-2G) had long-term radical scavenging activity against DPPH. The reaction of AA-2G or 6-Acyl-AA-2G with DPPH was very slow when compared with AA. However, one molecule of these derivatives consumed approximately three molecules of DPPH radicals at the end of the experiment (2 h). In contrast, one molecule of AA scavenged two molecules of DPPH radicals, and the reaction ended in the short time (<10 min). The quantity of radicals quenched by AA-2G and 6-Acyl-AA-2G was superior to that of AA in a long-term reaction.