A compact fiber-integrated optofluidic platform for highly specific microRNA Forster resonance energy transfer detection
A compact fiber-integrated optofluidic platform for highly specific microRNA Forster resonance energy transfer detection
复制标题
用于高度特异性 microRNA Forster 共振能量转移检测的紧凑型光纤集成光流控平台
作者：
Feng Hongtao;Liu Lin;Chen Yi;Shu Weiliang;Huang Yuqing;Zhang Baoyue;Wu Tianzhun;Jin Zongwen;Chen Yan
microRNA（miRNAs）作为一种有前途的疾病生物标志物，引起了人们的广泛兴趣。然而，miRNA的定量测量在生物化学研究中提出了重大挑战。在这项工作中，我们开发了一种创新的光流体平台，使用Förster共振能量转移（FRET）原理进行快速，简单，定量和高特异性的miRNA检测。提出了一种新的三向连接FRET探针，以实现快速和无酶的miRNA检测。利用该平台，我们实现了一步式、免扩增、设备操作简单的miRNA检测，并实现了低浓度的miRNA鉴定。该检测系统可实现对三碱基错配的高特异性区分，且样品体积显著减少，有利于材料有限样品中低水平miRNA的检测。一个紧凑、低成本、高灵敏度和选择性的miRNA分析平台的建立为床旁诊断提供了有价值的工具。
MicroRNAs (miRNAs) have attracted extensive interest as promising biomarkers for the profiling of diseases. However, quantitative measurement of miRNAs presents a significant challenge in biochemical studies. In this work, we developed an innovative optofluidic platform to perform a rapid, simple, quantitative and high-specificity miRNA assay using the Förster resonance energy transfer (FRET) principle. A novel three-way junction FRET probe was proposed to enable rapid and enzyme-free miRNA detection. Using this platform, we performed one-step, amplification-free miRNA detection with simple device operation and achieved miRNA identification at a low concentration. The detection system could achieve high specificity for discrimination of three-base mismatches, and the sample volume was significantly reduced, favorable for low-level miRNA detection in material-limited samples. The establishment of a compact, low-cost, highly sensitive and selective miRNA analysis platform provides a valuable tool for point-of-care diagnosis.