A ferroxidation/permeation iron uptake system is required for virulence in Ustilago maydis
A ferroxidation/permeation iron uptake system is required for virulence in Ustilago maydis
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DOI：
10.1105/tpc.106.043588
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发表时间：
2006-11-01
期刊：
影响因子：
11.6
通讯作者：
Kahmann, Regine
中科院分区：
文献类型：
--
作者：
Eichhorn, Heiko;Lessing, Franziska;Kahmann, Regine
在玉米黑粉菌中，严格调节的cAMP信号级联是致病性发展所必需的。转录组分析使用全基因组微阵列建立，以确定推定的靶基因的蛋白激酶A催化亚基Adr 1揭示了9个基因与推定的功能，在两个高亲和力的铁吸收系统。这些基因位于不同染色体上的三个基因簇中，包括先前鉴定的参与铁载体生物合成的互补铁载体营养缺陷型基因R11和R12。所有九个基因加上三个额外的基因与基因簇的转录也通过Urbs 1转录因子铁共调节。两个组成部分的高亲和力铁吸收系统的特点更详细：fer 2，编码高亲和力的铁通透酶;和fer 1，编码铁多铜氧化酶。Fer 2定位于质膜，并补充了缺乏高亲和力铁通透酶的酿酒酵母ftr 1突变体。在病原菌的发育过程中，fer 2的表达仅限于菌丝在植株内部的增殖阶段。fer 2和fer 1缺失突变体的毒力受到强烈影响。这些数据突出了通过铁渗透酶和多铜氧化酶的高亲和力铁吸收系统在美国的活体营养发育中的重要性。玉米/玉米（Zea mays）致病系统。
In the smut fungus Ustilago maydis, a tightly regulated cAMP signaling cascade is necessary for pathogenic development. Transcriptome analysis using whole genome microarrays set up to identify putative target genes of the protein kinase A catalytic subunit Adr1 revealed nine genes with putative functions in two high-affinity iron uptake systems. These genes locate to three gene clusters on different chromosomes and include the previously identified complementing siderophore auxotroph genes sid1 and sid2 involved in siderophore biosynthesis. Transcription of all nine genes plus three additional genes associated with the gene clusters was also coregulated by iron through the Urbs1 transcription factor. Two components of a high-affinity iron uptake system were characterized in more detail: fer2, encoding a high-affinity iron permease; and fer1, encoding an iron multicopper oxidase. Fer2 localized to the plasma membrane and complemented an ftr1 mutant of Saccharomyces cerevisiae lacking a high-affinity iron permease. During pathogenic development, fer2 expression was confined to the phase of hyphal proliferation inside the plant. fer2 as well as fer1 deletion mutants were strongly affected in virulence. These data highlight the importance of the high-affinity iron uptake system via an iron permease and a multicopper oxidase for biotrophic development in the U. maydis/maize (Zea mays) pathosystem.