MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients
MUC5AC expression is increased in bronchial submucosal glands of stable COPD patients
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DOI：
10.1111/j.1365-2559.2009.03377.x
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发表时间：
2009-09-01
期刊：
影响因子：
6.4
通讯作者：
Papi, Alberto
中科院分区：
文献类型：
--
作者：
Caramori, Gaetano;Casolari, Paolo;Papi, Alberto
目的：黏液咳痰是慢性阻塞性肺疾病(COPD)的常见特征。MUC5AC和MUC5B是主要的粘蛋白，主要从中央呼吸道的粘膜下腺释放。方法：对10例非吸烟者、20例肺功能正常吸烟者和20例COPD吸烟者的支气管环进行研究。采用高碘酸-席夫(PAS)和阿尔新兰组织化学染色，MUC5AC和MUC5B免疫组织化学染色，并对免疫反应面积进行定量。COPD组支气管黏膜下腺MUC5AC+细胞面积[20%(5.5-31.7%)]高于吸烟组[9.5%(2.5-17.5%)]和非吸烟组[2%(0.4-6.2%)；P<；0.05]。吸烟组(COPD组和非吸烟组)MUC5AC+细胞在支气管表面上皮细胞的面积[73.5%(25-92%)]较不吸烟组[15%(2.7-32%)]显著增加(P<0.01)。腺体大小、PAS、Alcian蓝染色和MUC5B表达在组间无显著差异。MUC5AC的表达与气流阻塞程度相关。结论：COPD患者黏膜下腺MUC5AC表达增加，提示MUC5AC可能参与了COPD的病理生理过程。
Aims:Mucus expectoration is a common feature of chronic obstructive pulmonary disease (COPD). MUC5AC and MUC5B, the major mucins, are released predominantly from submucosal glands in the central airways. The aim was to investigate gland size and MUC5AC and MUC5B expression in bronchial rings from smokers with COPD compared with control groups.Methods and results:Bronchial rings from 10 non-smoking subjects, 20 smokers with normal lung function and 20 smokers with COPD were studied. Periodic acid-Schiff (PAS) and Alcian blue histochemistry and MUC5AC and MUC5B immunohistochemistry followed by quantification of the immunoreactive area was performed. The area occupied by MUC5AC+ cells in bronchial submucosal glands was increased in COPD [20% (5.5-31.7%) gland area] compared with smokers with normal lung function [9.5% (2.5-17.5%); P < 0.05] and non-smoking subjects [2% (0.4-6.2%); P < 0.05]. The area occupied by MUC5AC+ cells in the bronchial surface epithelium was also increased in smokers (with/without COPD) [73.5% (25-92%) epithelial area] compared with non-smoking subjects [15% (2.7-32%); P < 0.01]. Gland size, PAS, Alcian blue staining and MUC5B expression were not significantly different among groups. MUC5AC expression correlated with the degree of airflow obstruction. MUC5AC and MUC5B expression correlated with pack-years.Conclusions:COPD is associated with increased MUC5AC expression in submucosal glands, indicating that MUC5AC may be involved in the pathophysiology of the disease.