Experimental Study on ATA Prescription in Treating Monkey with Chronic Infection of Simian Immunodeficiency Virus
Experimental Study on ATA Prescription in Treating Monkey with Chronic Infection of Simian Immunodeficiency Virus
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发表时间：
2005
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通讯作者：
Fu Linchun
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作者：
Fu Linchun
[目的]探讨中药复方ATA对猴免疫缺陷病毒(SIV)慢性感染的治疗作用。[方法]用SIVmac251感染恒河猴8只，建立SIV慢性感染猴模型。18个月后随机分为模型组和ATA组，分别给予生理盐水和ATA 2g·kg-1·d-1灌胃56d。观察治疗前后体征变化，用流式细胞仪检测血浆T淋巴细胞亚群CD4+、CD8+水平，光镜下观察淋巴结活检情况。[结果]ATA组治疗后体重增加，感染性腹泻发生率下降，差异无统计学意义。ATA对白细胞计数和血浆SIV复制数无明显影响，但可升高血浆T淋巴细胞亚群CD4+、CD8+水平(与模型组比较，P<0.05)。两组治疗前均有淋巴结变性、衰竭，治疗后ATA组出现淋巴滤泡增生、生发中心扩张、免疫细胞增多等淋巴结重建。[结论]ATA对SIV慢性感染猴免疫功能的恢复重建有一定作用，但抗病毒作用不明显。
[Objective] To investigate the effect of ATA, a herbal medicine compound, for the treatment of the chronic infection of simian immunodeficiency virus ( SIV) in monkeys. [ Methods ] Eight Rhesus monkeys were infected with SIVmac251 to establish monkey models with chronic infection of SIV. After 18 months, the monkeys were randomized into model group and ATA group, administered with saline and ATA 2 g·kg-1·d-1 for 56 days respectively. The changes of signs were observed, plasma levels of T-lymphocyte subsets CD4+ and CD8+ were detected by flow cytometry and the lymph node biopsy were observed under light microscope before and after treatment. [Results] After treatment, the body weight increased and the incidence of infective diarrhea decreased in ATA group, the difference being insignificant. ATA had no obvious effect on white blood cells counting and the replication number of plasma SIV, but increased the plasma levels of T-lymphocyte subsets CD4+ and CD8+(P0.05 compared with the model group). Before treatment, the degeneration and exhaustion of lymph node existed in the two groups; however, there appeared the reconstruction of lymph node such as lymph follicle proliferating, germinal center expanding and immune cells growing in ATA group after treatment. [Conclusion] ATA has a certain effect on the recovery and reconstruction of immune function in monkeys with chronic infection of SIV but has no obvious anti-virus effect.