Geometric constraints alter cell arrangements within curved epithelial tissues.
Geometric constraints alter cell arrangements within curved epithelial tissues.
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DOI：
10.1091/mbc.e17-01-0060
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发表时间：
2017-12-01
影响因子：
3.3
通讯作者：
Saunders TE
中科院分区：
文献类型：
--
作者：
Rupprecht JF;Ong KH;Yin J;Huang A;Dinh HH;Singh AP;Zhang S;Yu W;Saunders TE
在弯曲的环境中，几何约束会导致细胞对空间的竞争。提取细胞几何形状的定量信息，以探索柱状细胞如何适合果蝇胚胎的弯曲前部。细胞变形和细胞重排使得能够在该高度弯曲的区域中进行包装。器官和组织的形成是复杂的三维过程，涉及细胞分裂、生长、迁移和重排，所有这些都发生在物理限制的区域内。然而，在体内三维分析这样的过程是具有挑战性的。在这里，我们专注于细胞化的过程中，在早期果蝇胚胎的前极，探讨细胞如何竞争的几何约束下的空间。使用微流控技术结合荧光显微镜，我们提取的三维上皮细胞形态的定量信息。我们观察到细胞膜重排，其中细胞交换邻居沿着顶-基底轴。这种顶部到底部的邻居交流观察到更频繁的前极比胚胎干。此外，细胞内的前极偏向躯干沿着其长轴相对于胚胎表面，与腹侧的最大偏斜。建立了曲面环境中细胞的顶点模型。我们可以在野生型胚胎和具有扭曲形态的胚胎中再现观察到的细胞偏斜。此外，这种建模表明，与圆柱形几何形状相比，椭圆形几何形状更可能发生细胞重排。总体而言，我们表明，几何约束可以影响三维上皮组织内的细胞形态和包装。
In curved environments, geometric constraints can result in cellular competition for space. Quantitative information about cell geometry was extracted to explore how columnar-like cells fit within the curved anterior of the Drosophila embryo. Cell deformations and cell rearrangements enable packing in this highly curved region. Organ and tissue formation are complex three-dimensional processes involving cell division, growth, migration, and rearrangement, all of which occur within physically constrained regions. However, analyzing such processes in three dimensions in vivo is challenging. Here, we focus on the process of cellularization in the anterior pole of the early Drosophila embryo to explore how cells compete for space under geometric constraints. Using microfluidics combined with fluorescence microscopy, we extract quantitative information on the three-dimensional epithelial cell morphology. We observed a cellular membrane rearrangement in which cells exchange neighbors along the apical-basal axis. Such apical-to-basal neighbor exchanges were observed more frequently in the anterior pole than in the embryo trunk. Furthermore, cells within the anterior pole skewed toward the trunk along their long axis relative to the embryo surface, with maximum skew on the ventral side. We constructed a vertex model for cells in a curved environment. We could reproduce the observed cellular skew in both wild-type embryos and embryos with distorted morphology. Further, such modeling showed that cell rearrangements were more likely in ellipsoidal, compared with cylindrical, geometry. Overall, we demonstrate that geometric constraints can influence three-dimensional cell morphology and packing within epithelial tissues.