Pathogenesis and transmission of SARS-CoV-2 in golden hamsters
Pathogenesis and transmission of SARS-CoV-2 in golden hamsters
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DOI：
10.1038/s41586-020-2342-5
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发表时间：
2020-05-14
期刊：
影响因子：
64.8
通讯作者：
Yen, Hui-Ling
中科院分区：
文献类型：
--
作者：
Sia, Sin Fun;Yan, Li-Meng;Yen, Hui-Ling
SARS-CoV-2在金黄色（叙利亚）仓鼠中的致病性和传播性与COVID-19在人类患者中的特征相似，这表明这些仓鼠可用于模拟这种疾病。（SARS-CoV-2），一种与SARS-CoV和在马蹄蝙蝠中检测到的SARS相关冠状病毒具有高度核苷酸同一性的新型冠状病毒，已经蔓延到世界各地，并对医疗保健系统和经济产生了全球性影响（1，2）。需要合适的小动物模型来支持疫苗和疗法的开发。在这里，我们报告的发病机制和传播的SARS-CoV-2在金黄色（叙利亚）仓鼠（Mesocricetus auratus）。免疫组化结果显示，SARS-CoV-2感染后第2、5天鼻粘膜、支气管上皮细胞和肺实变区出现病毒抗原，第7天病毒迅速清除，肺泡上皮细胞增生。我们还在十二指肠上皮细胞中发现了病毒抗原，并在粪便中检测到病毒RNA。值得注意的是，SARS-CoV-2通过直接接触和气溶胶从接种的仓鼠有效地传播给未接种的仓鼠。通过污染笼子中的污染物传播的效率不高。虽然在接种仓鼠的鼻洗液中连续检测到病毒RNA 14天，但传染期很短，与感染性病毒的检测相关，而不是病毒RNA。接种和自然感染的仓鼠在接种后或接触后第6-7天表现出明显的体重减轻;所有仓鼠在14天内恢复至其原始体重并产生中和抗体。我们的研究结果表明，与SARS-CoV-2感染金黄仓鼠的特征相似，在人类轻度SARS-CoV-2感染中发现。
The pathogenicity and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters resemble features of COVID-19 in human patients, suggesting that these hamsters could be used to model this disease.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies(1,2). A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (Mesocricetus auratus). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6-7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.