Towards organ printing: engineering an intra-organ branched vascular tree.
Towards organ printing: engineering an intra-organ branched vascular tree.
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DOI：
10.1517/14712590903563352
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发表时间：
2010-03
影响因子：
4.6
通讯作者：
Mironov V
中科院分区：
文献类型：
--
作者：
Visconti RP;Kasyanov V;Gentile C;Zhang J;Markwald RR;Mironov V
厚的三维组织工程构建物的有效血管化是组织工程中的一个问题。与天然器官一样，组织工程化的器官内血管树必须由分层分支血管段的网络组成。尽管有这样的要求，目前的组织工程的努力仍然主要集中在工程大直径的大血管或微血管网络。我们提出了器官打印或器官内分支血管树的机器人添加剂生物制品的新兴概念，基于血管组织球体进行自组装的能力。分析了这种基于器官打印技术的机器人生物制造方法用于组织工程的器官内血管化的可行性和挑战，所述器官打印技术使用自组装血管组织球体，包括临床相关的血管细胞来源。不可能在没有有效血管化的情况下设计3D厚组织或器官构造。器官内的血管系统不能通过简单的大血管和微血管的连接来建立。成功地工程化适合于外科植入的功能性人体器官将需要伴随工程化的"内置"器官内分支血管系统。器官打印使得能够利用“内置”的器官内分支血管树来生物制造人类器官构造。
Effective vascularization of thick three-dimensional engineered tissue constructs is a problem in tissue engineering. As in native organs, a tissue-engineered intra-organ vascular tree must be comprised of a network of hierarchically branched vascular segments. Despite this requirement, current tissue-engineering efforts are still focused predominantly on engineering either large-diameter macrovessels or microvascular networks. We present the emerging concept of organ printing or robotic additive biofabrication of an intra-organ branched vascular tree, based on the ability of vascular tissue spheroids to undergo self-assembly. The feasibility and challenges of this robotic biofabrication approach to intra-organ vascularization for tissue engineering based on organ-printing technology using self-assembling vascular tissue spheroids including clinically relevantly vascular cell sources are analyzed. It is not possible to engineer 3D thick tissue or organ constructs without effective vascularization. An effective intra-organ vascular system cannot be built by the simple connection of large-diameter vessels and microvessels. Successful engineering of functional human organs suitable for surgical implantation will require concomitant engineering of a ‘built in’ intra-organ branched vascular system. Organ printing enables biofabrication of human organ constructs with a ‘built in’ intra-organ branched vascular tree.