Oxidized proteins in plasma of patients with heart failure: Role in endothelial damage
Oxidized proteins in plasma of patients with heart failure: Role in endothelial damage
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DOI：
10.1016/j.ejheart.2008.01.016
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发表时间：
2008-03-01
影响因子：
18.2
通讯作者：
Agostoni, PierGiuseppe
中科院分区：
文献类型：
--
作者：
Banfi, Cristina;Brioschi, Maura;Agostoni, PierGiuseppe
目的：鉴定慢性心衰患者血浆中氧化蛋白的含量，探讨氧化蛋白在血管内皮细胞损伤中的可能作用。方法：用免疫分析法检测40例心衰患者和20例年龄匹配的正常人血浆中氧化蛋白的水平，并用蛋白组学方法检测血浆中氧化蛋白的水平。结果：心力衰竭患者血浆氧化蛋白水平明显高于对照组(P<0.05)。
Background: Oxidative stress is increased in the failing heart, and this might contribute to the pathogenesis of myocardial remodelling and heart failure (HF).Aim: To identify the oxidized proteins in plasma of chronic HF patients and to evaluate their possible role in endothelial damage.Methods: Plasma levels of oxidized proteins were measured by immunoassay and by analysis in albumin and immumoglobulin depleted plasma using a proteomic approach, in 40 HF patients and in 20 age-matched normal subjects. Analysis of the effects of proteins oxidized in vitro on human endothelial cell (EC) viability was also performed.Results: Plasma levels of oxidized proteins were significantly higher in HF patients than in controls (p