Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats
Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats
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DOI：
10.1007/s12020-010-9419-0
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发表时间：
2011-06-01
期刊：
影响因子：
3.7
通讯作者：
Wen, Gebo
中科院分区：
文献类型：
--
作者：
Li, Qiangxiang;Ao, Xiang;Wen, Gebo
本研究旨在探讨氨基胍和维生素C（VitC）对糖尿病肾病大鼠IV型胶原的影响。腹腔注射链脲佐菌素（STZ）建立糖尿病肾病大鼠模型。将大鼠随机分为5组：正常对照组（n = 10）、糖尿病组（n = 10）、氨基胍组（n = 10）、VitC组（n = 10）、氨基胍+VitC组（n = 10）。16周后检测各组大鼠一般情况、血糖、糖化血红蛋白、血尿素氮、血肌酐、血清IV型胶原、尿白蛋白排泄率、肌酐清除率，免疫组化法检测IV型胶原蛋白，原位杂交法检测肾脏IV型胶原α 1 mRNA的表达。结果表明：（1）糖尿病组、氨基胍组、VitC组、VitC+氨基胍组均出现糖尿病及肾脏病变;（2）氨基胍+VitC能改善糖尿病肾病大鼠的一般情况，降低血尿素氮、血肌酐、尿白蛋白排泄率，提高肌酐清除率。与糖尿病组相比，治疗组的IV型胶原表达显著下调。氨基胍和维生素C分别通过抑制IV型胶原的表达来保护糖尿病肾病的肾脏病变，而氨基胍和维生素C对它们具有协同作用。
The aim of this article is to investigate the effects of Aminoguanidine and vitamin C (VitC) on type IV collagen in diabetic nephropathy rats. Diabetic nephropathy rats were induced by intraperitoneal injection of STZ. Rats were randomly divided into five groups: normal control group (n = 10), diabetes group (n = 10), aminoguanidine group (n = 10), VitC group (n = 10), aminoguanidine and VitC group (n = 10). After 16 weeks, the general conditions, blood gloucose, glycosylated hemoglobin, blood urea nitrogen, serum creatinine, serum type IV collagen, urinary albumin excretion rate, and creatinine clearance rate were detected, type IV collagen protein was determined by immunohistochemical analysis as well as the expression of collagen type IV alpha 1 mRNA were determined by in situ hybridization analysis in the kidneys of each group. The results were (1) diabetes mellitus and renal lesions occurred in the diabetes group, aminoguanidine group, VitC group, VitC and aminoguanidine group; (2) aminoguanidine and VitC improved the general conditions of diabetic nephropathy rats, decreased blood urea nitrogen, serum creatinine, and urinary albumin excretion rate as well as increased creatinine clearance rate. The expressions of collagen type IV were significantly down-regulated in treatment groups in contrast to the diabetes group. Aminoguanidine and VitC protect renal lesions in diabetic nephropathy, respectively, by inhibiting expression of type IV collagen, while aminoguanidine and VitC have a synergistic effect on them.