Apoptotic anticancer activity of a novel fatty alcohol ester isolated from cultured marine diatom, Phaeodactylum tricornutum
Apoptotic anticancer activity of a novel fatty alcohol ester isolated from cultured marine diatom, Phaeodactylum tricornutum
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DOI：
10.1016/j.jff.2013.10.011
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发表时间：
2014-01-01
影响因子：
5.6
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
--
作者：
Samarakoon, Kalpa W.;Ko, Ju-Young;Jeon, You-Jin
一种新型脂肪醇酯；从培养的海洋硅藻三角褐指藻 Bohlin 中分离出五种化合物，其中鉴定出 8-乙酰氧基-6-甲基辛酸壬酯 (NAMO)。通过筛选三种不同的癌细胞系，包括人早幼粒细胞白血病（HL-60）、人肺癌（A549）和小鼠黑色素瘤（B16F10），检查了NAMO的抗癌作用。在体外观察到HL-60对癌细胞生长有强烈的抑制作用，与其他癌细胞相比，其IC50值为65.151μM。 NAMO 在 HL-60 细胞中的凋亡发生表现为亚 G(1) 期 DNA 的积累和核浓缩的剂量依赖性方式。从蛋白表达结果可以看出，在凋亡途径上，NAMO通过促凋亡蛋白和抗凋亡蛋白的凋亡诱导控制，激活Bax并抑制Bcl-xL，并通过上调另一种凋亡诱导剂caspase-3和p53来开启细胞凋亡。 (C) 2013 Elsevier Ltd. 保留所有权利。
A novel fatty alcohol ester; nonyl 8-acetoxy-6-methyloctanoate (NAMO) was identified among the five isolated compounds from cultured marine diatom, Phaeodactylum tricornutum Bohlin. The anticancer effects of NAMO were examined through screening of three different cancer cell lines including a human promyelocytic leukemia (HL-60), a human lung carcinoma (A549) and a mouse melanoma (B16F10). A strong suppression of cancer cells growth was observed in HL-60 and its IC50 value was 65.151 mu M compared to the other cancer cells in vitro. The apoptotic occurrence in HL-60 cells by NAMO was evidenced as the accumulation of DNA in sub-G(1) phase and nuclear condensations dose-dependent manner. From the protein expression results it was revealed on the apoptotic pathway that NAMO switched on the apoptosis by activation of Bax and suppression of Bcl-xL through apoptotic inducing control of pro- and anti-apoptotic proteins, and by up-regulation of another inducers of apoptosis, caspase-3 and p53. (C) 2013 Elsevier Ltd. All rights reserved.