Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
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DOI：
10.1128/jvi.05879-11
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发表时间：
2012-04-01
影响因子：
5.4
通讯作者：
Krueger, Detlev H.
中科院分区：
文献类型：
--
作者：
Klempa, Boris;Witkowski, Peter T.;Krueger, Detlev H.
我们发现了第一种非洲本土汉坦病毒，桑加苏病毒(SANGV)。该病毒是从困在西非几内亚森林栖息地的一只非洲林鼠(Hylmyscus Simus)身上分离出来的。在这里，我们报告了该病毒的基因和功能特性的特征。测定了SANGV的全基因组，显示出典型的汉坦病毒结构。小(S)、中(M)和大(L)基因组片段包括编码核衣壳蛋白、两个包膜糖蛋白和病毒聚合酶的基因，分别长1,746，3,650和6,531个核苷酸。确定了SANGV基因组所有三个片段的5‘和3’末端，并预测形成了典型的布尼亚病毒的狭长柄结构。对所有三个片段序列的系统发育分析证实，SANGV是一种与Murinae相关的汉坦病毒，与欧洲多布拉瓦-贝尔格莱德病毒关系最密切。然而，我们发现SANGV使用β(1)整合素而不是β(3)整合素和衰变加速因子(DAF)/CD55作为进入受体。此外，我们还发现SANGV能在I型干扰素缺陷的Vero E6细胞中强烈诱导III型lambda干扰素(IFN-lambda)表达。这些特性在与小鼠流感病毒相关的汉坦病毒中是独一无二的，使该病毒在专注于汉坦病毒发病机制的比较研究中有用。
We have discovered the first indigenous African hantavirus, Sangassou virus (SANGV). The virus was isolated from an African wood mouse (Hylomyscus simus), trapped in a forest habitat in Guinea, West Africa. Here, we report on the characterization of the genetic and functional properties of the virus. The complete genome of SANGV was determined and showed typical hantavirus organization. The small (S), medium (M), and large (L) genome segments containing genes encoding nucleocapsid protein, two envelope glycoproteins, and viral polymerase were found to be 1,746, 3,650, and 6,531 nucleotides long, respectively. The exact 5 ' and 3 ' termini for all three segments of the SANGV genome were determined and were predicted to form the panhandle structures typical of bunyaviruses. Phylogenetic analyses of all three segment sequences confirmed SANGV as a Murinae-associated hantavirus most closely related to the European Dobrava-Belgrade virus. We showed, however, that SANGV uses beta(1) integrin rather than beta(3) integrin and decay-accelerating factor (DAF)/CD55 as an entry receptor. In addition, we demonstrated a strong induction of type III lambda interferon (IFN-lambda) expression in type I IFN-deficient Vero E6 cells by SANGV. These properties are unique within Murinae-associated hantaviruses and make the virus useful in comparative studies focusing on hantavirus pathogenesis.