Revised classification of the DLA loci by serological studies.
Revised classification of the DLA loci by serological studies.
复制标题
通过血清学研究修订 DLA 位点分类。
作者：
K. Krumbacher;M. J. van der Feltz;M. Happel;C. Gerlach;L. K. Lösslein;H. Grosse
用兔抗犬β 2微球蛋白和山羊抗兔免疫球蛋白处理DLA型犬外周血单核细胞，使DLA I类分子在细胞膜（溶酶带）上聚集。利用70种确定的DLA-A和DLA-B抗血清，用这种方法处理的淋巴细胞仅对单特异性DLA-A血清的补体依赖性溶解具有抗性，而DLA-B抗血清的反应性未被阻断；相反，所有hla - b抗血清均能完全淋巴溶解。因此，显然与β 2微球蛋白无关的hla - b抗原被指定为II类基因产物的候选物。由于溶酶带对hla - c抗血清的不同反应，目前还不能对该抗原系列进行精确的定位。
Peripheral blood mononuclear cells from DLA typed dogs were treated with rabbit-anti-dog-beta 2-microglobulin and subsequently with goat-anti-rabbit-immunoglobulin in order to aggregate the DLA class I molecules on the cell membrane (lysostrip). Utilizing a panel of 70 defined DLA-A and DLA-B antisera, lymphocytes treated in this way showed resistance to complement dependent lysis with monospecific DLA-A sera only, whereas reactivity of DLA-B antisera was not blocked; on the contrary, complete lympholysis with each DLA-B antiserum was recognized. Thus, the DLA-B antigens, evidently not associated with beta 2-microglobulin, are designated as candidates for class II gene products. The different reactions of DLA-C antisera after lysostrip did not allow a precise assignment of this antigen series as yet.