CLONING OF HUMAN ANDROGEN RECEPTOR COMPLEMENTARY-DNA AND LOCALIZATION TO THE X-CHROMOSOME
CLONING OF HUMAN ANDROGEN RECEPTOR COMPLEMENTARY-DNA AND LOCALIZATION TO THE X-CHROMOSOME
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DOI：
10.1126/science.3353727
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发表时间：
1988-04-15
期刊：
影响因子：
56.9
通讯作者：
WILSON, EM
中科院分区：
文献类型：
--
作者：
LUBAHN, DB;JOSEPH, DR;WILSON, EM
雄激素受体（AR）介导雄性类固醇的作用。利用核受体家族DNA结合域的一致核苷酸序列，从流分类的人类X染色体文库中克隆出人类AR基因组DNA。AR基因定位在人类X染色体着丝粒和q13之间。克隆的互补DNA，用AR特异性寡核苷酸探针选择，在猴肾（COS）细胞中表达，并产生高亲和力的雄激素结合蛋白，具有与天然AR相对应的类固醇结合特异性。在已知含有AR的人、大鼠和小鼠组织中鉴定出9.6千碱基的优势信使RNA物种，而在缺乏AR雄激素结合活性的组织中，包括雄激素不敏感小鼠的肾脏和肝脏，无法检测到。推断出的AR在dna结合域内的氨基酸序列与孕酮受体的序列一致性最高。
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors. The AR gene was localized on the human X chromosome between the centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, was expressed in monkey kidney (COS) cells and yielded a high-affinity androgen-binding protein with steroid-binding specificity corresponding to that of native AR. A predominant messenger RNA species of 9.6 kilobases was identified in human, rat, and mouse tissues known to contain AR and was undetectable in tissues lacking AR androgen-binding activity, including kidney and liver from androgen-insensitive mice. The deduced amino acid sequence of AR within the DNA-binding domain has highest sequence identity with the progesterone receptor.