Photoreceptor organisation and phenotypic characterization in retinas of two diurnal rodent species: Potential use as experimental animal models for human vision research
Photoreceptor organisation and phenotypic characterization in retinas of two diurnal rodent species: Potential use as experimental animal models for human vision research
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DOI：
10.1016/j.visres.2007.08.011
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发表时间：
2008-02-01
期刊：
影响因子：
1.8
通讯作者：
Hicks, David
中科院分区：
文献类型：
--
作者：
Bobu, Corina;Lahmam, Mohamed;Hicks, David
为了表征两种昼夜活动的鼠样啮齿动物的视杆和视锥分布和组成，我们使用多种视杆和视锥特异性抗体对成年Arvicanthis ansorgei和Lemniscomys barbarus的视网膜进行免疫组织化学处理。检测的抗体包括视紫红质、视锥蛋白、泛抑制蛋白和视锥蛋白、恢复蛋白和cGMP依赖离子通道。在这两个物种中，视网膜由大约33%的视锥细胞组成，大多数抗体的染色模式相似。数据显示，这两种昼夜活动的啮齿动物拥有大量的锥体，以严格的解剖阵列组织。这表明，白天活动的啮齿动物通常具有较高的锥体数量，可以为研究锥体病理生理学提供有价值的模型。(c) 2007 Elsevier Ltd.版权所有。
To characterize rod and cone distribution and composition in two diurnal mouse-like rodents, retinas from adult Arvicanthis ansorgei and Lemniscomys barbarus were processed for immunohistochemistry using multiple rod- and cone-specific antibodies. Antibodies tested included rhodopsin, cone opsins, pan-arrestin and cone arrestin, recoverin, and cGMP dependent ion channel. In both species, retinas were composed of approximately 33% cones, and most antibodies gave similar staining patterns. Data show these two diurnal rodents possess large numbers of cones, organised in a strict anatomical array. This suggests that diurnal rodents in general possess elevated cone numbers and could constitute valuable models for investigating cone pathophysiology. (c) 2007 Elsevier Ltd. All rights reserved.