Nodal marginal zone B-cell lymphomas may arise from different subsets of marginal zone B lymphocytes.
Nodal marginal zone B-cell lymphomas may arise from different subsets of marginal zone B lymphocytes.
复制标题
淋巴结边缘区 B 细胞淋巴瘤可能源自边缘区 B 淋巴细胞的不同亚群。
作者：
A. Conconi;F. Bertoni;E. Pedrinis;T. Motta;E. Roggero;S. Luminari;C. Capella;M. Bonato;F. Cavalli;E. Zucca
淋巴结边缘区b细胞淋巴瘤（MZL）是一种罕见且未被广泛研究的实体，约占所有非霍奇金淋巴瘤的2%。对8例淋巴结性MZL患者的免疫球蛋白重链可变区（V(H)）基因的互补决定区2、3 （CDR2、CDR3）进行PCR扩增、克隆和测序。所有的都显示出潜在的功能性V(H)重排。V(H)基因家族的使用是无偏的，没有任何特定的V(H)基因或基因家族的过度代表。检测到体细胞V(H)突变的存在，在8例患者中有6例与最接近的种系序列偏差从4%到17%不等。在3个突变中，替换与沉默突变的比率表明存在抗原选择过程。对相同克隆PCR产物的不同亚克隆进行测序，可以检测4例分析患者的克隆内变异性。观察到的V(H)突变模式表明，淋巴结MZL，以前被认为是记忆B细胞的恶性肿瘤，可能来自不同的边缘区B细胞亚群（表达未突变的V(H)基因的幼稚B细胞），来自显示体细胞突变而无克隆内变异的记忆B细胞，以及生发中心B细胞，它们具有经历体细胞超突变过程的能力。(血。2001;98:781 - 786)
Nodal marginal zone B-cell lymphoma (MZL) is a rare and not extensively studied entity that accounts for approximately 2% of all non-Hodgkin lymphomas. Complementarity-determining regions 2 and 3 (CDR2, CDR3) of the immunoglobulin heavy-chain variable region (V(H)) genes were amplified by polymerase chain reaction (PCR), cloned, and sequenced in 8 patients with nodal MZL. All showed a potentially functional V(H) rearrangement. The use of V(H) gene families was unbiased and without overrepresentation of any particular V(H) gene or gene family. The presence of somatic V(H) mutations was detected, with a deviation from the closest germ line sequence ranging from 4% to 17% in 6 of 8 patients. In 3 mutations, the replacement-to-silent mutation ratio suggested the presence of an antigen-selected process. Sequencing different subclones of the same cloned PCR products allowed the detection of intraclonal variability in 4 analyzed patients. The observed pattern of V(H) mutations suggested that nodal MZL, formerly deemed a malignancy of memory B cells, may arise from different subsets of marginal zone B cells-the naive B cells that express unmutated V(H) genes-from memory B cells showing somatic mutations without intraclonal variation, and from germinal center B cells defined by their capacity to undergo the somatic hypermutation process. (Blood. 2001;98:781-786)
脾和 MALT 型原发性淋巴结边缘区淋巴瘤。
DOI：
10.1097/00000478-199901000-00006
发表时间：
1999
期刊：
The American journal of surgical pathology
影响因子：
--
作者：
Campo,E;Miquel,R;Krenacs,L;Sorbara,L;Raffeld,M;Jaffe,ES
通讯作者：
Jaffe,ES
影响因子：
20.3
作者：
R. Damle;T. Wasil;F. Fais;F. Ghiotto;A. Valetto;S. L. Allen;A. Buchbinder;D. Budman;K. Dittmar;J. Kolitz;S. Lichtman;P. Schulman;V. Vinciguerra;K. Rai;M. Ferrarini;N. Chiorazzi
通讯作者：
R. Damle;T. Wasil;F. Fais;F. Ghiotto;A. Valetto;S. L. Allen;A. Buchbinder;D. Budman;K. Dittmar;J. Kolitz;S. Lichtman;P. Schulman;V. Vinciguerra;K. Rai;M. Ferrarini;N. Chiorazzi
影响因子：
20.3
作者：
FISHER, RI;DAHLBERG, S;GROGAN, TM
通讯作者：
GROGAN, TM