Alteration of pregnancy related hormones and fetal survival in F-344 rats exposed by inhalation to benzo(a)pyrene
Alteration of pregnancy related hormones and fetal survival in F-344 rats exposed by inhalation to benzo(a)pyrene
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DOI：
10.1016/s0890-6238(02)00058-8
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发表时间：
2002-11-01
影响因子：
3.3
通讯作者：
Nyanda, AM
中科院分区：
文献类型：
--
作者：
Archibong, AE;Inyang, F;Nyanda, AM
本研究的目的是评价亚急性吸入苯并(A)芘(BaP)对胎鼠存活和黄体维持的影响。在将孕鼠分配到治疗组和对照组之前，在妊娠第8天通过中腹切开确定着床位的数目。随后，动物被随机分配到三个处理组和两个对照组。治疗包括亚急性吸入苯并苯25、75和100 mg/m(3)，每天4h，连续10天(GD-11-20)。对照动物要么假暴露于炭黑(CB)中以控制惰性BaP载体，要么保持不暴露(UNC)。分别于妊娠第15天和第17天经窦-眶静脉穿刺法采集血浆标本。产后测定每窝产仔数，胎儿存活率表示为相应植入部位的百分比。放射免疫测定法用于测定血浆孕酮、雌激素和催乳素(间接测量蜕膜促黄体素)浓度。与CB(每窝96.7%)和UNC(每窝98.9%)相比，BaP处理组大鼠的胚胎存活率(25µg/m(3)，每窝78.3%；75µg/m(3)，每窝38.0%；100µg/m(3)，每窝33.8%；P<；0.05)呈剂量依赖性下降。与对照组相比，亚急性暴露于BaP的大鼠的血浆孕酮、雌激素和催乳素浓度也有所下降。这些数据表明，吸入苯并苯降低了胎儿存活率，从而降低了暴露动物的促黄体活性。(C)2002 Elsevier Science Inc.保留所有权利。
The objective of this study was to evaluate the effect of subacute exposure to inhaled benzo(a)pyrene (BaP) on fetal survival and luteal maintenance using timed-pregnant Fisher 344 rats. Prior to assignment of pregnant rats to treatment and control groups, numbers of implantation sites were determined on gestation day (GD) 8 via midventral laparotomy. Subsequently, animals were assigned randomly to three treatment groups and two control groups. Treatment consisted of subacute exposure of rats via inhalation to BaP 25, 75, and 100 mug/m(3), 4 h daily for 10 days (GD-11-20). Control animals were either sham exposed to carbon black (CB) to control for inert BaP carrier or remained unexposed (UNC). Blood samples were collected on days 15 and 17 of gestation via sinus orbital veini-puncture for plasma. Number of pups per litter was determined postpartum and fetal Survival rate was expressed as a percentage of the corresponding implantation sites. Radioinummoassays were used to determine plasma progesterone, estrogen, and prolactin (indirect measurement of decidual luteotropin) concentrations. Fetal survival among BaP-treated rats declined in a dose-dependent manner (25 mug/m(3), 78.3% per litter; 75 mug/m(3), 38.0% per litter; 100 mug/m(3), 33.8% per litter; P < 0.05) compared with CB (96.7% per litter) and UNC (98.9% per litter). Plasma progesterone, estrogen, and prolactin concentrations also declined as a result of subacute exposure of rats to BaP compared to controls. These data suggest that inhaled BaP compromised fetal survival and consequently luteotropic activity in the exposed animals. (C) 2002 Elsevier Science Inc. All rights reserved.