Genetic analysis of Drosophila melanogaster polytene chromosome region 44D-45F: loci required for viability and fertility.
Genetic analysis of Drosophila melanogaster polytene chromosome region 44D-45F: loci required for viability and fertility.
复制标题
果蝇多线染色体区域 44D-45F 的遗传分析：活力和生育力所需的位点。
DOI：
10.1093/genetics/160.4.1503
复制
发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Boswell,RobertE
中科院分区：
文献类型：
--
作者：
Mohr,StephanieE;Boswell,RobertE
进行了遗传筛查，以识别2号染色体右臂45 A区域中参与果蝇卵子发生的基因突变。以前在P-元件插入或利用X射线或EMS作为诱变剂的筛选中已经确定了该区域的几个致死性但没有雌性不育突变。在这里，我们报告的EMS诱导的突变，在21个基本位点的45 D-45 F区域，包括13个以前未确定的位点的鉴定。此外，我们分离出三个突变等位基因的一个新发现的基因座所需的生育能力，无产阶级。在精子个体化时或个体化之前，突变会破坏精子发生，并导致卵子发生的多种缺陷，包括生殖细胞包囊的不适当分裂和卵子发生在第4阶段的停滞。
A genetic screen to identify mutations in genes in the 45A region on the right arm of chromosome 2 that are involved in oogenesis in Drosophila was undertaken. Several lethal but no female sterile mutations in the region had previously been identified in screens forP-element insertion or utilizing X rays or EMS as a mutagen. Here we report the identification of EMS-induced mutations in 21 essential loci in the 45D–45F region, including 13 previously unidentified loci. In addition, we isolated three mutant alleles of a newly identified locus required for fertility,sine prole. Mutations insine proledisrupt spermatogenesis at or before individualization of spermatozoa and cause multiple defects in oogenesis, including inappropriate division of the germline cyst and arrest of oogenesis at stage 4.