A 90-day subchronic toxicity study of nivalenol, a trichothecene mycotoxin, in F344 rats
A 90-day subchronic toxicity study of nivalenol, a trichothecene mycotoxin, in F344 rats
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DOI：
10.1016/j.fct.2007.07.005
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Hirose, Masao
中科院分区：
文献类型：
--
作者：
Takahashi, Miwa;Shibutani, Makoto;Hirose, Masao
在喂食含0、6.25、25或100 ppm浓度饲料的雄性和雌性F344大鼠中进行了雪腐镰刀菌烯醇（NIV）（一种雪花莲真菌毒素）的亚慢性毒性研究，持续90天。从实验开始，在100 ppm剂量下观察到两种性别动物的体重减轻和稀便，从第6周开始，在25 ppm剂量下也观察到雄性动物的体重减轻。尸检时，在100 ppm剂量下，两种性别动物的许多器官显示绝对重量降低，主要是由于身体生长减慢，雌性动物的相对胸腺重量也明显降低。在血液学上，雄性动物在100 ppm时发现白色血细胞计数降低，雌性动物在6.25 ppm时发现。此外，在100 ppm剂量下检测到两种性别动物的血小板计数、雄性动物的红细胞计数和雌性动物的血红蛋白浓度降低。组织学上，在100 ppm剂量下，主要在两种性别动物的造血和免疫器官以及垂体前叶和雌性生殖器官中观察到给药相关变化，如胸腺萎缩、骨髓细胞减少、嗜碱性细胞弥漫性肥大伴垂体前叶去势细胞增加和卵巢闭锁卵泡增加。根据血液学数据，确定NIV的无明显不良作用水平低于6.25 ppm（雄性和雌性均为0.4 mg/kg体重/天）。(c)2007爱思唯尔有限公司保留所有权利。
A subchronic toxicity study of nivalenol (NIV), a trichothecene mycotoxin, was conducted in male and female F344 rats fed diet containing 0, 6.25, 25 or 100 ppm concentration for 90 days. Decrease of body weight and loose stools were observed at 100 ppm in both sexes from the start of the experiment, and body weight reduction was also observed at 25 ppm in males from week 6. At necropsy, many organs demonstrated reduced absolute weights at 100 ppm in both sexes, mostly due to the reduction in the body growth, with reduction of relative thymus weight also being evident in females. Hematologically, decrease of the white blood cell count was found at 100 ppm in males and from 6.25 ppm in females. In addition, decreased platelet counts in both sexes, red blood cell counts in males, and the hemoglobin concentration in females were detected at 100 ppm. Histopathologically, treatment-related changes were predominantly observed in the hematopoietic and immune organs and the anterior pituitary in both sexes and female reproductive organs at 100 ppm, such as thymic atrophy, hypocellularity in the bone marrow, diffuse hypertrophy of basophilic cells with increase of castration cells in the anterior pituitary, and increase of ovarian atretic follicles. Based on the hematological data, the no-observed-adverse-effect level of NIV was determined to be less than 6.25 ppm (0.4 mg/kg body weight/day for both males and females). (c) 2007 Elsevier Ltd. All rights reserved.