Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences
Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences
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DOI：
10.1016/j.diagmicrobio.2006.12.019
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发表时间：
2007-06-01
影响因子：
2.9
通讯作者：
Ezaki, Takayuki
中科院分区：
文献类型：
--
作者：
Nhung, Pham Hong;Ohkusu, Kiyofumi;Ezaki, Takayuki
利用来自27个肠杆菌属93个种的165株肠杆菌的部分dnaJ序列分析了肠杆菌科的系统发育关系。dnaJ系统发育与16S rDNA序列构建的系统发育基本一致，但dnaJ系统树比16S rDNA系统树获得的单系群更多。dnaJ基因的分歧程度约为16S rDNA的6倍。此外，与tuf和atpD基因相比，dnaJ基因显示出最大的区分能力，通过dnaJ序列分析促进了任何2种肠杆菌的明确区分。通过将72株临床分离株分配到正确的肠杆菌属种，证实了dnaJ序列在鉴定中的应用。我们的数据表明，dnaJ基因序列的分析可以作为一个强大的标记，在家庭肠杆菌科的物种水平上的系统发育研究和鉴定。(c)2007年爱思唯尔公司All rights reserved.
Phylogenetic relations within the family Enterobacteriaceae were analyzed using partial dnaJ sequences of 165 strains belonging to 93 species from 27 enterobacterial genera. The dnaJ phylogeny was in relative agreement with that constructed by 16S rDNA sequences, but more monophyletic groups were obtained from the dnaJ tree than from the 16S rDNA tree. The degree of divergence of the dnaJ gene was approximately 6 times greater than that of 16S rDNA. Also, the dnaJ gene showed the most discriminatory power in comparison with tuf and atpD genes, facilitating clear differentiation of any 2 enterobacterial species by dnaJ sequence analysis. The application of dnaJ sequences to the identification was confirmed by assigning 72 clinical isolates to the correct enterobacterial species. Our data indicate that analysis of the dnaJ gene sequences can be used as a powerful marker for phylogenetic study and identification at the species level of the family Enterobacteriaceae. (c) 2007 Elsevier Inc. All rights reserved.