Chinchilla and murine models of upper respiratory tract infections with respiratory syncytial virus
Chinchilla and murine models of upper respiratory tract infections with respiratory syncytial virus
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DOI：
10.1128/jvi.79.10.6035-6042.2005
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发表时间：
2005-05-01
影响因子：
5.4
通讯作者：
Durbin, JE
中科院分区：
文献类型：
--
作者：
Gitiban, N;Jurcisek, JA;Durbin, JE
呼吸道合胞病毒（RSV）是婴儿和老年人下呼吸道感染的主要原因。虽然原发性感染是最严重的，但终生上呼吸道再感染是规律。虽然对上呼吸道RSV感染或宿主对RSV的粘膜免疫了解相对较少，但最近的文献表明RSV是诱发细菌性中耳炎（OM）的主要病毒病原体。在此，我们描述了鼻咽和耳咽管RSV感染的小鼠和栗鼠模型。两种啮齿类宿主在鼻内激发后对上呼吸道RSV感染易感;然而，灰鼠被证明比小鼠更容易感染。灰鼠模型将可能是非常有用的测试RSV在细菌OM中的作用和RSV疫苗候选物的效力，旨在提供粘膜和细胞毒性T淋巴细胞免疫。最终，我们希望研究这些候选物的相对能力，以潜在地防止病毒易感性细菌OM。
Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in infants and the elderly. While the primary infection is the most serious, reinfection of the upper airway throughout life is the rule. Although relatively little is known about either RSV infection of the upper respiratory tract or host mucosal immunity to RSV, recent literature suggests that RSV is the predominant viral pathogen predisposing to bacterial otitis media (OM). Herein, we describe mouse and chinchilla models of RSV infection of the nasopharynx and Eustachian tube. Both rodent hosts were susceptible to RSV infection of the upper airway following intranasal challenge; however, the chinchilla proved to be more permissive than the mouse. The chinchilla model will likely be extremely useful to test the role of RSV in bacterial OM and the efficacy of RSV vaccine candidates designed to provide mucosal and cytotoxic T-lymphocyte immunity. Ultimately, we hope to investigate the relative ability of these candidates to potentially protect against viral predisposal to bacterial OM.