Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography.
Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography.
复制标题
通过逆流色谱法用水性两相溶剂系统纯化蛋白质。
DOI：
10.1080/10826069808010130
复制
发表时间：
1998
影响因子：
2.9
通讯作者：
Y. Ito
中科院分区：
文献类型：
--
作者：
Y. Shibusawa;Y. Ito
摘要 水性聚合物相系统由 Albertsson1 在 20 世纪 50 年代提出，已用于分离各种大分子和细胞颗粒，包括蛋白质、2-6 核酸、7-10 细胞器、11 病毒、12,13 细菌细胞、14,15 等。使用这些溶剂系统，已经提出了几种不同的方法来进行生物样品的纯化，例如单步分离、1 重复批量萃取、逆流分配(CCD)16,17 和逆流色谱 (CCC).18-21 在这些技术中，CCC 被认为在分配效率和分离时间方面最有效。
Abstract Aqueous polymer phase systems, introduced by Albertsson1 in the 1950s, have been used for partitioning a variety of macromolecules and cell particles which include proteins,2-6 nucleic acid,7-10 cell organelles,11 viruse,12,13 bacterial cells,14,15 etc. Using these solvent systems, several different approaches have been made for performing purification of biological samples, such as single step partitioning,1 repetitive batch extraction, countercurrent distribution (CCD)16,17 and countercurrent chromatography (CCC).18-21 Among these techniques, CCC is considered to be most effective in terms of partition efficiency and separation times.