A gene encoding a polydnavirus structural polypeptide is not encapsidated
A gene encoding a polydnavirus structural polypeptide is not encapsidated
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DOI：
10.1006/viro.2000.0248
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发表时间：
2000-04-10
期刊：
影响因子：
3.7
通讯作者：
Webb, BA
中科院分区：
文献类型：
--
作者：
Deng, LQ;Stoltz, DB;Webb, BA
多DNA病毒是与一些寄生蜂相关的共生病毒，其作为原病毒在黄蜂基因组内垂直传播。为了研究这种共生关系，编码丰富的Campoletis sonorensis多DNA病毒病毒体蛋白的基因的特征在于。该基因不存在于黄蜂基因组中，只在病毒复制期间表达。免疫定位研究检测到编码的44 kDa蛋白质仅在输卵管组织与超微结构研究检测表位之间或病毒粒子包膜。44-kDa蛋白的表达和定位与其作为病毒结构蛋白是一致的，但是该基因仅在黄蜂基因组内的定位是非典型的，这提高了该蛋白在病毒体组装过程中偶然包装的可能性。为了解决这种可能性，进行了定量斑点印迹和基因组Southern印迹杂交，以确定在复制过程中p44基因的拷贝数是否不成比例地增加，正如对编码病毒体蛋白的基因所预期的那样。p44基因的拷贝数在支持病毒复制的组织中增加，但在其他组织中不变，表明该基因在复制细胞中扩增。这些数据表明，编码多DNA病毒病毒粒子蛋白的基因可能分布在黄蜂和寄生虫病毒基因组之间。(C)北京大学出版社.
Polydnaviruses are symbiotic viruses associated with some parasitic Hymenoptera that are vertically transmitted as proviruses within wasp genomes. To study this symbiotic association a gene encoding an abundant Campoletis sonorensis polydnavirus Virion protein was characterized. This gene is not encapsidated but resides in the wasp genome where it is expressed only during virus replication. Immunolocalization studies detected the encoded 44-kDa protein only in oviduct tissue with ultrastructural studies detecting epitopes between or on virion envelopes. Expression and localization of the 44-kDa protein are consistent with its being a viral structural protein but localization of the gene only within the wasp genome is atypical, raising the possibility that this protein is adventitiously packaged during virion assembly. To address this possibility, quantitative dot blot and genomic Southern blot hybridizations were performed to determine whether the copy number of the p44 gene Increased disproportionately during replication, as would be expected for a gene encoding a virion protein. The copy number of the p44 gene increases in tissues supporting virus replication but is unchanged In other tissues, suggesting that this gene is amplified in replicative cells. The data indicate that genes encoding polydnavirus virion proteins may be distributed between wasp and encapsidated viral genomes. (C) 2000 Academic Press.