Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo.
Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo.
复制标题
柔红霉素和藤黄酸共载半胱胺-CdTe量子点在体外和体内最大限度地减少淋巴瘤的多药耐药性
DOI：
10.2147/ijn.s115037
复制
发表时间：
2016
影响因子：
8
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Zhou Y;Wang R;Chen B;Sun D;Hu Y;Xu P
为减少柔红霉素（DNR）治疗恶性淋巴瘤的毒副作用和多药耐药（MDR），开发了一种半胱胺修饰的碲化镉（Cys-CdTe）量子点与柔红霉素和藤黄酸（GA）纳米粒共载的化疗制剂（DNR GA-Cys-CdTe NPs）。评价了纳米粒的物理性质、载药效率和释药行为，并采用3-[4，5-二甲基噻唑-2-基]-2，5-二苯基四氮唑溴化物法考察了纳米粒的细胞毒性。这些DNR-GA-Cys-CdTe纳米粒具有pH响应性，对多药耐药淋巴瘤Raji/DNR细胞具有剂量依赖性的抗增殖活性。流式细胞仪检测DNR在细胞内的蓄积，Western blotting检测P-gp在Raji/DNR细胞内的表达，表明DNR-GA-Cys-CdTe纳米粒能降低Raji/DNR细胞的MDR。这种多药递送系统将是一个有前途的策略，以减少对淋巴瘤的MDR。
To minimize the side effects and the multidrug resistance (MDR) arising from daunorubicin (DNR) treatment of malignant lymphoma, a chemotherapy formulation of cysteamine-modified cadmium tellurium (Cys-CdTe) quantum dots coloaded with DNR and gambogic acid (GA) nanoparticles (DNR-GA-Cys-CdTe NPs) was developed. The physical property, drug-loading efficiency and drug release behavior of these DNR-GA-Cys-CdTe NPs were evaluated, and their cytotoxicity was explored by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide assay. These DNR-GA-Cys-CdTe NPs possessed a pH-responsive behavior, and displayed a dose-dependent antiproliferative activity on multidrug-resistant lymphoma Raji/DNR cells. The accumulation of DNR inside the cells, revealed by flow cytometry assay, and the down-regulated expression of P-glycoprotein inside the Raji/DNR cells measured by Western blotting assay indicated that these DNR-GA-Cys-CdTe NPs could minimize the MDR of Raji/DNR cells. This multidrug delivery system would be a promising strategy for minimizing MDR against the lymphoma.