Significantly different proliferative potential of oral mucosal epithelial cells between six animal species
Significantly different proliferative potential of oral mucosal epithelial cells between six animal species
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DOI：
10.1002/jbm.a.34849
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发表时间：
2014-06-01
影响因子：
4.9
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Kondo, Makoto;Yamato, Masayuki;Okano, Teruo
近年来，再生医学发展迅速。特别是，由于口腔粘膜上皮细胞可以无创地获得，因此培养的上皮细胞片已被用于许多异位移植。此外，实验动物培养细胞特性的验证加速了再生医学的应用。本研究比较了6种动物口腔黏膜上皮细胞的特性。人和猪的上皮相对较厚。大鼠细胞的集落形成效率最高，其次是狗、人、兔和猪，而小鼠细胞的集落都是平行克隆的，在集落形成实验中不可计数。我们还发现兔和猪的细胞增殖很差，没有喂食层就不能形成细胞片。相比之下，即使在没有喂食层和霍乱毒素的情况下，当细胞播种密度高时，培养的狗和小鼠细胞形成连续的薄片。这些结果表明，口腔粘膜上皮细胞的种间变异是相当大的，必须根据预期用途选择特定的实验动物或人类细胞。(c) 2013 Wiley Periodicals, Inc.；[J]中国生物医学工程学报，2014,31(2):379 - 379。
There has been an upsurge in regenerative medicine in recent years. In particular, because oral mucosal epithelial cells can be obtained noninvasively, cultured epithelial cell sheets have been used in a number of ectopic transplantations. Additionally, the verification of the properties of experimental animals' cultured cells has accelerated the application of regenerative medicine. In the present study, the properties of oral mucosal epithelial cells were compared between six animal species. The human and pig epithelia were relatively thicker than the epithelia of the other species. The colony-forming efficiency of the rat was the highest, followed by those of the dog, human, rabbit, and pig, whereas the colonies of the mouse cells were all paraclone and uncountable in the colony-forming assay. We also found that the rabbit and pig cells proliferated poorly and were unable to form cell sheets without feeder layers. In contrast, even in the absence of feeder layers and cholera toxin, cultured dog and mouse cells formed contiguous sheets, when the cell seeding density was high. These results indicate that interspecies variation is considerable in oral mucosal epithelial cells and that specific experimental animal or human cells must be chosen according to the intended use. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1829-1837, 2014.