Intracellular Staphylococcus aureus Control by Virulent Bacteriophages within MAC-T Bovine Mammary Epithelial Cells
Intracellular Staphylococcus aureus Control by Virulent Bacteriophages within MAC-T Bovine Mammary Epithelial Cells
复制标题
MAC-T 牛乳腺上皮细胞内有毒噬菌体对细胞内金黄色葡萄球菌的控制。
DOI：
10.1128/aac.01990-16
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发表时间：
2017-02-01
影响因子：
4.9
通讯作者：
Wang, Ran
中科院分区：
文献类型：
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作者：
Zhang, Lili;Sun, Lichang;Wang, Ran
众所周知，噬菌体可以有效地杀死细胞外繁殖的细菌。本研究证明噬菌体能穿透牛乳腺上皮细胞，并以时间依赖的方式清除细胞内的金黄色葡萄球菌。尤其是VBSAUMJS25噬菌体在孵育后3h内即可到达胞核。噬菌体的内吞效率为12%。这种杀死细胞内宿主细菌的能力表明了基于噬菌体的治疗的实用性，并可能保护患者免受复发感染和治疗失败的影响。
Bacteriophages (phages) are known to effectively kill extracellular multiplying bacteria. The present study demonstrated that phages penetrated bovine mammary epithelial cells and cleared intracellular Staphylococcus aureus in a timedependent manner. In particular, phage vB_SauM_JS25 reached the nucleus within 3 h postincubation. The phages had an endocytotic efficiency of 12%. This ability to kill intracellular host bacteria suggests the utility of phage-based therapies and may protect patients from recurrent infection and treatment failure.