PRIMARY RESPIRATORY SYNCYTIAL VIRUS-INFECTION IN MICE
PRIMARY RESPIRATORY SYNCYTIAL VIRUS-INFECTION IN MICE
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DOI：
10.1002/jmv.1890260207
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发表时间：
1988-10-01
影响因子：
12.7
通讯作者：
KARZON, DT
中科院分区：
文献类型：
--
作者：
GRAHAM, BS;PERKINS, MD;KARZON, DT
描述了呼吸道合胞病毒（RSV）感染的小鼠模型。高滴度、大体积接种物导致RSV在BALB/c小鼠肺中复制至高滴度。大于15周龄的小鼠更容易感染RSV。在32周龄小鼠中可达到高达106.9空斑形成单位（pfu）/克肺的滴度。年老的小鼠经历临床疾病，表现为皮毛起皱、活动减少和体重减轻。与小于8周龄的小鼠相比，感染RSV的老年小鼠的肺组织学显示细支气管炎和肺泡腔中淋巴细胞和巨噬细胞数量增加。该模型将作为研究RSV感染恢复和保护的免疫决定因素的基础。
A mouse model of respiratory syncytial virus (RSV) infection is described. A high-titered, large-volume inoculum results in replication of RSV to a high titer in lungs of BALB/c mice. Mice older than 15 weeks of age are more susceptible to RSV infection. Titers up to 106.9 plaque-forming units (pfu)/gram lung can be attained in 32-week-old mice. Older mice expeience a clinical illness manifested by ruffled fur, reduced activity, and weight loss. Lung histology of older mice infected with RSV shows bronchiolitis and increased number of lymphocytes and macrophages in alveolar spaces compared with that of mice less than 8 weeks old. This model will serve as the basis for investigating immunodeterminants of recovery and protection from RSV infection.