A Degraded Fragment of HIV-1 Gp120 in Rat Hepatocytes Forms Fibrils and Enhances HIV-1 Infection
A Degraded Fragment of HIV-1 Gp120 in Rat Hepatocytes Forms Fibrils and Enhances HIV-1 Infection
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大鼠肝细胞中 HIV-1 gp120 的降解片段形成原纤维并增强 HIV-1 感染
DOI：
10.1016/j.bpj.2017.08.005
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发表时间：
2017-10-01
影响因子：
3.4
通讯作者：
Li, Lin
中科院分区：
文献类型：
--
作者：
Chen, Jinquan;Ren, Ruxia;Li, Lin
确定增强艾滋病毒感染的宿主或病毒因素对于预防艾滋病毒的性传播至关重要。来源于人类精液的淀粉样纤维，包括精液来源的病毒感染增强剂和精液凝胶，在体外显著增强HIV-1感染。在这项研究中，我们报道了一个短降解肽片段1（DPF 1）来自天然HIV-1包膜蛋白gp 120加载大鼠肝细胞，形成纤维通过自组装，从而增强HIV-1感染，促进HIV-1的结合到靶细胞。此外，DPF 1形成的纤维可能被用作杂交种子，以加速精液来源的病毒感染增强子和精液凝胶纤维的形成。这将有助于阐明影响HIV-1感染的病毒因素。DPF 1作为gp 120的类似物，含有CD 4结合的关键残基，可能有助于HIV疫苗的设计和HIV进入抑制剂的开发。
Identification of the host or viral factors that enhance HIV infection is critical for preventing sexual transmission of HIV. Amyloid fibrils derived from human semen, including semen-derived enhancer of virus infection and semenogelins, enhance HIV-1 infection dramatically in vitro. In this study, we reported that a short-degraded peptide fragment 1 (DPF1) derived from native HIV-1 envelope protein gp120-loaded rat hepatocytes, formed fibrils by self-assembly and thus enhanced HIV-1 infection by promoting the binding of HIV-1 to target cells. Furthermore, DPF1-formed fibrils might be used as a crossing seed to accelerate the formation of semen-derived enhancer of virus infection and semenogelin fibrils. It will be helpful to clarify the viral factors that affect HIV-1 infection. DPF1 as an analog of gp120 containing the critical residues for CD4 binding might be useful for designing of HIV vaccines and developing HIV entry inhibitors.