IMMORTALIZED RETINAL NEURONS DERIVED FROM SV40 T-ANTIGEN-INDUCED TUMORS IN TRANSGENIC MICE
IMMORTALIZED RETINAL NEURONS DERIVED FROM SV40 T-ANTIGEN-INDUCED TUMORS IN TRANSGENIC MICE
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DOI：
10.1016/0896-6273(90)90204-s
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发表时间：
1990-05-01
期刊：
影响因子：
16.2
通讯作者：
MESSING, A
中科院分区：
文献类型：
--
作者：
HAMMANG, JP;BAETGE, EE;MESSING, A
Immortalized retinal neurons have been established in tissue culture from retinal tumors arising in transgenic mice. The mice carry the SV40 T-antigen under the control of 5’flanking sequences from the human phenylethanolamine N-methyltransferase(PNMT) gene in order to target oncogene expression to adrenergic cell types. The retinal cultures contain a proliferating population of T-antigen-positive cells with a neuronal morphology that includes formation of extensive neuritic processes. We identified the cells as amacrine-derived neurons by immunofluorescence using the cell-specific monoclonal antibodies VC1. l and HPC-1. The cells also express all three neurofilament subunits and GAP-43. These results indicate that CNS neurons can be transformed in transgenic animals to generate cultured cells with many properties of mature neurons.
