PENTAMIDINE TRANSPORT AND SENSITIVITY IN BRUCEI-GROUP TRYPANOSOMES
PENTAMIDINE TRANSPORT AND SENSITIVITY IN BRUCEI-GROUP TRYPANOSOMES
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DOI：
10.1111/j.1550-7408.1976.tb03787.x
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
PATTON, CL
中科院分区：
文献类型：
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作者：
DAMPER, D;PATTON, CL
布氏锥虫血流型、培养型、亚种株和T.对这些生物体中喷他脒摄取的转运参数的分析显示了与药物敏感性相对应的差异。T. B.布鲁氏菌对药物具有高亲和力，并具有高摄取率，如[3H]喷他脒转运的KmandVmax值所示。尽管喷他脒和己烯巴脒抵抗与运动障碍有关。这种情况本身不赋予对喷他脒的抗性，也不影响喷他脒的转运。然而，耐药菌株显示与T一样的戊烷脒转运率较低。B.罗得西亚，其特征是对药物不太敏感。在所有研究的形式和菌株中，前环锥鞭毛体对喷他脒最不敏感，并且具有显著的排除药物的能力。
Sensitivity to pentamidine of bloodstream forms and culture forms ofTrypanosoma brucei brucei, strains of this subspecies, and strains ofT. brucei rhodesiensecharacteristically differsin vitro.Analyses of transport parameters for pentamidine uptake in these organisms show differences that correspond with drug sensitivity. Long slender bloodstream forms ofT. b. bruceihave a high affinity for the drug and high rates of uptake as indicated byKmandVmaxvalues for [3H]pentamidine transport. Although pentamidine and stilbamidine resistance is associated with dyskinetoplasty. this condition does not itself confer resistance to pentamidine nor does it affect pentamidine transport. However, drug‐resistant strains show lower rates for pentamidine transport as doesT. b. rhodesiense, which is characteristically less sensitive to the drug. Of all the forms and strains studied, procyclic trypomastigotes were least sensitive to pentamidine and had a remarkable ability to exclude the drug.